Aptamer-conjugated and drug-loaded acoustic droplets for ultrasound theranosis
Aptamer-conjugated and drug-loaded acoustic droplets for ultrasound theranosis
复制标题
DOI：
10.1016/j.biomaterials.2011.11.036
复制
发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Yeh, Chih-Kuang
中科院分区：
文献类型：
--
作者：
Wang, Chung-Hsin;Kang, Shih-Tsung;Yeh, Chih-Kuang
肿瘤治疗需要多功能的治疗策略，并有针对性地针对治疗药物，以减少全身毒性，提高疗效。在这项研究中，我们制备了适体结合和阿霉素(DOX)负载的声学液滴，其中包括液体全氟戊烷化合物和脂基壳层材料的核心。Sgc8c适配子的结合提供了特异性靶向CCRF-CEM细胞进行成像和治疗的能力。引入高强度聚焦超声(HIFU)来触发靶向声学液滴汽化(ADV)，通过惯性空化实现对癌细胞的机械性破坏，通过局部药物释放实现化学治疗。HIFU辐射显示，与非共轭液滴相比，适体共轭液滴的细胞活力下降了56.8%，是非共轭液滴的4.5倍。此外，与未汽化或部分汽化的液滴相比，完全汽化的液滴导致癌细胞摄取最高的DOX。光学研究清楚地显示了液滴靶向CEM细胞的ADV发生的瞬时变化，B型超声成像在超声图像中显示了ADV的对比增强。总之，我们制造的液滴作为一种混合的化学和机械策略，在提供超声成像能力的同时，可以特异性地破坏超声介导的ADV上的癌细胞。(C)2011爱思唯尔有限公司。保留所有权利。
Tumor therapy requires multi-functional treatment strategies with specific targeting of therapeutics to reduce general toxicity and increase efficacy. In this study we fabricated and functionally tested aptamer-conjugated and doxorubicin (DOX)-loaded acoustic droplets comprising cores of liquid perfluoropentane compound and lipid-based shell materials. Conjugation of sgc8c aptamers provided the ability to specifically target CCRF-CEM cells for both imaging and therapy. High-intensity focused ultrasound (HIFU) was introduced to trigger targeted acoustic droplet vaporization (ADV) which resulted in both mechanical cancer cell destruction by inertial cavitation and chemical treatment through localized drug release. HIFU insonation showed a 56.8% decrease in cell viability with aptamer-conjugated droplets, representing a 4.5-fold increase in comparison to non-conjugated droplets. In addition, the fully-vaporized droplets resulted in the highest DOX uptake by cancer cells, compared to non-vaporized or partially vaporized droplets. Optical studies clearly illustrated the transient changes that occurred upon ADV of droplet-targeted CEM cells, and B-mode ultrasound imaging revealed contrast enhancement by ADV in ultrasound images. In conclusion, our fabricated droplets functioned as a hybrid chemical and mechanical strategy for the specific destruction of cancer cells upon ultrasound-mediated ADV, while simultaneously providing ultrasound imaging capability. (C) 2011 Elsevier Ltd. All rights reserved.