Transgenic plant production mediated by Agrobacterium in indica rice
Transgenic plant production mediated by Agrobacterium in indica rice
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DOI：
10.1007/bf00232216
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发表时间：
1996-06-01
影响因子：
6.2
通讯作者：
Hinata, K
中科院分区：
文献类型：
--
作者：
Rashid, H;Yokoi, S;Hinata, K
利用农杆菌介导的转基因技术，建立了一个可重复的籼稻转基因植株的转化体系。三周龄的小盾片愈伤组织是一个很好的起始材料。用携带含有β-葡糖醛酸酶（GUS）和潮霉素抗性（HygR）基因的质粒pIG 121 Hm的根癌土壤杆菌菌株EHA 101感染这些菌株。潮霉素（50 mg/l）用作选择剂。在农杆菌悬浮液和共培养基中加入乙酰丁香酮（50 μ M）被证明是成功转化所必需的。Basmati 370的转化效率为22%，与已报道的粳稻和双子叶植物的转化效率相当。获得了大量形态正常、可育的转基因植株。Southern杂交分析证实外源基因已整合到转基因植株的基因组中。GUS和HygR基因在R1代中得到了遗传和表达。孟德尔分离在一些R1后代。
A reproducible system has been developed for the production of transgenic plants in indica rice using Agrobacterium-mediated gene transfer. Three-week-old scutella calli served as an excellent starting material. These were infected with an Agrobacterium tumefaciens strain EHA101 carrying a plasmid pIG121Hm containing genes for beta-glucuronidase (GUS) and hygromycin resistnace(HygR). Hygromycin (50 mg/l) was used as a selectable agent. Inclusion of acetosyringone (50 mu M) in the Agrobacterium suspension and co-culture media proved to be indispensable for successful transformation. Transformation efficiency of Basmati 370 was 22% which was as high as reported in japonica rice and dicots. A large number of morphologically normal, fertile transgenic plants were obtained. Integration of foreign genes into the genome of transgenic plants was confirmed by Southern blot analysis. GUS and HygR genes were inherited and expressed in R1 progeny. Mendelian segregation was observed in some R1 progeny.