TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.
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DOI：
10.1016/j.jped.2013.12.012
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发表时间：
2014-09
影响因子：
3.3
通讯作者：
A. Redondo;M. E. Ceccon;A. L. Silveira-Lessa;C. Quinello;P. Palmeira;W. B. Carvalho;M. Carneiro-Sampaio
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文献类型：
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作者：
A. Redondo;M. E. Ceccon;A. L. Silveira-Lessa;C. Quinello;P. Palmeira;W. B. Carvalho;M. Carneiro-Sampaio
目的分析迟发性败血症新生儿单核细胞Toll样受体(TLR)-2和TLR-4的表达。10名新生儿(37%)培养呈阳性。外周血单核细胞TLR-2、TLR-4表达和中位荧光强度(MFI)采用免疫分型法检测，并用BD FACSDiva流式细胞仪(Becton，Dickinson and Company，USA)进行分析。结果37%的败血症新生儿有微生物存在，且均有高水平的促炎细胞因子(IL-8、IL-6、IL-1β)和抗炎细胞因子(IL-10)证实炎症/败血症过程。在单核细胞中，TLR-4在感染新生儿中的表达频率较高(p=0.01)。结论本研究探讨了败血症新生儿的天然免疫反应。几乎完全依赖先天免疫系统的败血症新生儿在体内单核细胞激活时几乎没有反应，这表明免疫反应受损，感染易感性增加。
ObjectiveTo analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.MethodsThis prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.ResultsMicroorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).ConclusionThis study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.