Isolation and identification of phytochemicals and biological activities of Hericium ernaceus and their contents in Hericium strains using HPLC/UV analysis.
Isolation and identification of phytochemicals and biological activities of Hericium ernaceus and their contents in Hericium strains using HPLC/UV analysis.
复制标题
DOI：
10.1016/j.jep.2016.02.038
复制
发表时间：
2016-05
影响因子：
5.4
通讯作者：
Dong Gu Lee;Hee-Wan Kang;Chun-Geon Park;Y. Ahn;Y. Shin
中科院分区：
文献类型：
--
作者：
Dong Gu Lee;Hee-Wan Kang;Chun-Geon Park;Y. Ahn;Y. Shin
民族药理学相关性 猴头菇在中医中自古以来就被用于治疗消化不良、胃溃疡和精神衰弱。材料与方法从H. ernaceus中分离得到Hericenone C、D、F。 ernaceus 通过开放柱色谱法进行鉴定，并根据 1 H NMR、13 C NMR 和 MS 等光谱分析进行鉴定。通过 HPLC/UV 分析测定猴头菇菌株中猴头菇酮 C、D 和 F 的含量。为了研究猴头菇提取物的抗炎作用，用 200 μg/mL 猴头菇提取物处理 RAW 264.7 细胞 48 小时。采用MTT法检测细胞生长情况。结果从H. ernaceus通过开放柱色谱法。通过 1 H NMR、 13 C NMR 和 MS 等光谱分析，将其结构阐明为猴头酮 C、D 和 F。通过 HPLC/UV 分析测定猴头菌菌株中猴头菇酮 C、D 和 F 的含量。猴头菇酮 C、D 和 F 含量分别在 Norugungdenglee-2 (8.289±0.593 mg/g)、KFRI-1453 (4.657±0.462 mg/g) 和 KFRI-1093 (5.408±0.420 mg/g) 菌株中最高。测试的所有猴头菇菌株提取物均抑制脂多糖和干扰素 γ 诱导的 RAW264.7 细胞的炎症活性。与对照相比，菌株KFRI-1093减少了约39.6%的NO生成。结论我们认为KFRI-1093的抗炎作用是由于山楂酮F含量所致。我们的结果有助于验证传统用途、天然药物和保健品。同时，所开发的简单、准确、快速的液相色谱方法可用于测定其他猴头菌菌株中猴头菇酮的含量。
Ethnopharmacological relevanceHericium ernaceushas been traditionally used for the treatment of dyspepsia, gastric ulcer and enervation in traditional Chinese medicine for a long time.Aim of the studyTo examine the effect ofHericiumstrains on their ability to inhibit LPS and interferon-γ induced NO production in cell culture and the bioassay correlation of hericenone C, D, F, isolated fromH. ernaceus.Materials and methodsHericenone C, D, F were isolated fromH. ernaceusby open column chromatography and identified on the basis of spectroscopic analyses including1H NMR,13C NMR and MS. The amounts of hericenone C, D, and F inHericiumstrains were determined by HPLC/UV analysis. In order to investigate the anti-inflammatory effect ofHericiumstrains extracts, RAW 264.7 cells were treated with 200 μg/mL ofHericiumstrains extracts for 48 h. Cell growth was assessed by MTT assay.ResultsPhytochemical constituents were isolated fromH. ernaceusby open column chromatography. Their structures were elucidated as hericenones C, D, and F on the basis of spectroscopic analyses including1H NMR,13C NMR and MS. The amounts of hericenones C, D, and F inHericiumstrains were determined by HPLC/UV analysis. Hericenones C, D, and F contents were highest in Norugungdenglee-2 (8.289±0.593 mg/g), KFRI-1453 (4.657±0.462 mg/g), and KFRI-1093 (5.408±0.420 mg/g) strains, respectively. AllHericiumstrains extracts tested inhibited the lipopolysaccharide- and interferon-γ-induced inflammatory activity of RAW264.7 cells. The strain KFRI-1093 about 39.6% reduced NO generation with compared to control.ConclusionWe believe that the anti-inflammatory effect of KFRI-1093 was due to hericenone F content. Our results contribute towards validation of the traditional use, natural drugs and health supplements. And also, the developed simple, accurate and rapid LC method can be used determinate the content of hericenones from otherHericiumstrains.