A Customized Gene Expression Microarray Reveals That the Brittle Stem Phenotype fs2 of Barley Is Attributable to a Retroelement in the HvCesA4 Cellulose Synthase Gene
A Customized Gene Expression Microarray Reveals That the Brittle Stem Phenotype fs2 of Barley Is Attributable to a Retroelement in the HvCesA4 Cellulose Synthase Gene
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DOI：
10.1104/pp.110.158329
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发表时间：
2010-08-01
期刊：
影响因子：
7.4
通讯作者：
Fincher, Geoffrey B.
中科院分区：
文献类型：
--
作者：
Burton, Rachel A.;Ma, Gang;Fincher, Geoffrey B.
大麦（Hordeum vulgare）脆茎突变体fs2（命名为X054和M245）与它们的亲本品系Ohichi和Shiroseto相比具有降低的结晶纤维素水平。一个定制设计的微阵列，基于长寡核苷酸技术，包括参与细胞壁代谢的基因，揭示了很少的基因的转录水平被改变的伸长区的茎节间，但这些包括一个显着减少的mRNA的HvCesA4纤维素合成酶基因的两个突变体。与此相反，丰富的几百个成绩单的变化，在上部，成熟区的茎节间，这大概反映了多效性的反应，削弱细胞壁，导致从主要的遗传病变。HvCesA4基因的测序揭示了两个突变株系的HvCesA4基因的第一内含子中存在一个964 bp的Copia样逆转录元件的长末端重复序列。逆转录因子似乎干扰HvCesA4基因的转录或mRNA的加工，这可能是突变体结晶纤维素含量较低和茎强度较低的原因。HvCesA4基因定位于大麦染色体1H上的一个位置，该位置与先前报道的fs2位置相一致。
The barley (Hordeum vulgare) brittle stem mutants, fs2, designated X054 and M245, have reduced levels of crystalline cellulose compared with their parental lines Ohichi and Shiroseto. A custom-designed microarray, based on long oligonucleotide technology and including genes involved in cell wall metabolism, revealed that transcript levels of very few genes were altered in the elongation zone of stem internodes, but these included a marked decrease in mRNA for the HvCesA4 cellulose synthase gene of both mutants. In contrast, the abundance of several hundred transcripts changed in the upper, maturation zones of stem internodes, which presumably reflected pleiotropic responses to a weakened cell wall that resulted from the primary genetic lesion. Sequencing of the HvCesA4 genes revealed the presence of a 964-bp solo long terminal repeat of a Copia-like retroelement in the first intron of the HvCesA4 genes of both mutant lines. The retroelement appears to interfere with transcription of the HvCesA4 gene or with processing of the mRNA, and this is likely to account for the lower crystalline cellulose content and lower stem strength of the mutants. The HvCesA4 gene maps to a position on chromosome 1H of barley that coincides with the previously reported position of fs2.