Candida albicans Int1p interacts with the septin ring in yeast and hyphal cells
Candida albicans Int1p interacts with the septin ring in yeast and hyphal cells
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DOI：
10.1091/mbc.12.11.3538
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发表时间：
2001-11-01
影响因子：
3.3
通讯作者：
Berman, J
中科院分区：
文献类型：
--
作者：
Gale, C;Gerami-Nejad, M;Berman, J
在酵母和菌丝形态之间转换的能力是机会致病菌白色念珠菌的重要毒力因子。尽管在初始隔膜处形成的丝状环的出现动力学在酵母和形成菌丝（芽管）的细胞中不同（Soll和Mitchell，1983），但调节这种差异的分子机制尚不清楚。Int1p，a C.白念珠菌基因产物在其C末端与酿酒酵母Bud 4p相似，在菌丝形态发生中起作用。在这里，我们报告说，在S。在酿酒酵母中，Int 1 p表达导致高度极化的细胞生长，具有离域的几丁质和胞质分裂和芽位选择模式的缺陷，这些表型也在S. cerevisiae septin突变株。Int 1 p在S.酿酒酵母在极化细胞的周围产生了复杂的螺旋状结构，其中含有septins和Int 1 p。此外，Int 1 p与Cdc 11 p和Cdc 12 p隔蛋白共免疫沉淀，Cdc 12 p是建立和维持这些Int 1 p/隔蛋白螺旋所必需的。虽然Swe 1 p激酶参与了INT 1诱导的S.在酿酒酵母中，它不是形成异位Int 1 p/septin结构所必需的。拉角在白色念珠菌中，Int 1 p对轴向出芽模式起重要作用，并与Cdc 3 p隔蛋白共定位于酵母和假菌丝细胞的母芽颈环中。在诱导菌丝的条件下，Cdc 3 p和Int 1 p定位于母细胞和芽管交界处远端的环。因此，Int 1 p/septin环相对于母-子细胞连接的位置将酵母/假菌丝生长与C.白色念珠菌。
The ability to switch between yeast and hyphal morphologies is an important virulence factor for the opportunistic pathogen Candida albicans. Although the kinetics of appearance of the filamentous ring that forms at the incipient septum differ in yeast and cells forming hyphae (germ tubes) (Soll and Mitchell, 1983), the molecular mechanisms that regulate this difference are not known. Int1p, a C. albicans gene product with similarity in its C terminus to Saccharomyces cerevisiae Bud4p, has a role in hyphal morphogenesis. Here we report that in S. cerevisiae, Int1p expression results in the growth of highly polarized cells with delocalized chitin and defects in cytokinesis and bud-site selection patterns, phenotypes that are also seen in S. cerevisiae septin mutant strains. Expression of high levels of Int1p in S. cerevisiae generated elaborate spiral-like structures at the periphery of the polarized cells that contained septins and Int1p. In addition, Int1p coimmunoprecipitated with the Cdc11p and Cdc12p septins, and Cdc12p is required for the establishment and maintenance of these Int1p/septin spirals. Although Swe1p kinase contributes to INT1-induced filamentous growth in S. cerevisiae, it is not required for the formation of ectopic Int1p/septin structures. La C. albicans, Int1p was important for the axial budding pattern and colocalized with Cdc3p septin in a ring at the mother-bud neck of yeast and pseudohyphal cells. Under conditions that induce hyphae, both Cdc3p and Int1p localized to a ring distal to the junction of the mother cell and germ tube. Thus, placement of the Int1p/septin ring with respect to the mother-daughter cell junction distinguishes yeast/pseudohyphal growth from hyphal growth in C. albicans.