The healing of confined critical size cancellous defects in the presence of silk fibroin hydrogel
The healing of confined critical size cancellous defects in the presence of silk fibroin hydrogel
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DOI：
10.1016/j.biomaterials.2004.09.040
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发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Migliaresi, C
中科院分区：
文献类型：
--
作者：
Fini, M;Motta, A;Migliaresi, C
通过成骨细胞培养和植入兔股骨远端临界尺寸缺损后，研究了可注射丝素蛋白（SF）水凝胶的体外和体内行为。商业合成聚（D，L丙交酯-乙交酯）共聚物用作对照材料。通过测量LDH释放、细胞增殖（WST 1）、分化（ALP、OC）和合成活性（胶原蛋白I、TGF β 1、IL-6）评价体外生物相容性。通过测量骨小梁体积（BV/TV）、骨小梁厚度（Tb.Th）、骨小梁数量（Tb.N）、骨小梁间距（Tb.Sp）、骨矿沉积率（MAR）和骨形成率（BFR/B.Pm），观察骨缺损愈合率和骨缺损内新成骨质量。体外试验表明，与阴性对照相比，两种材料均显著增加了细胞增殖。与对照材料和阴性对照相比，发现SF水凝胶的TGF-β 1水平显著增加。当用于填充兔股骨中的临界尺寸缺损时，两种材料均促进骨愈合。与对照凝胶相比，SF水凝胶处理的缺损的新形成的骨显示出显著更高的BV/TV、Tb.Th、MAR和BFR/B.Pm以及更低的Tb.Sp值。在12周时，SF水凝胶处理的缺损的再生长的骨比对照合成聚合材料处理的缺损的再生长的骨更类似于正常骨，除了Tb.N值与正常骨的Tb.N值显著不同（p < 0.05）。与用合成聚合物材料处理的对照相比，SF水凝胶处理的缺损的MAR和BFR/B.Pm呈现显著（p < 0.05）更高的值，证实SF水凝胶加速重塑过程。(C)2004爱思唯尔有限公司保留所有权利。
In vitro and in vivo behaviour of an injectable silk fibroin (SF) hydrogel was studied through osteoblast cultures and after implantation in critical-size defects of rabbit distal femurs. A commercial synthetic poly(D,L lactide-glycolide) copolymer was used as control material. In vitro biocompatibility was evaluated by measuring LDH release, cell proliferation (WST1), differentiation (ALP, OC), and synthetic activity (collagen I, TGF beta1, IL-6). Bone defect healing rate and quality of the newly formed bone inside the defects were determined in vivo by measuring trabecular bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), mineral apposition rate (MAR) and bone formation rate (BFR/B.Pm). In vitro tests indicated that both materials significantly increased cell proliferation in comparison with the negative control. A significant increase in the TGF-beta1 level was found for SF hydrogel in comparison with the control material and negative control. Both materials promoted bone healing when used to fill critical size defects in rabbit femurs. The new-formed bone of the SF hydrogel treated defects showed significantly higher BV/TV, Tb.Th, MAR and BFR/B.Pm and lower Tb.Sp values in comparison with the control gel. At 12 weeks the re-grown bone of the SF hydrogel-treated defects appeared more similar to normal bone than that of the control synthetic polymeric material-treated defects, except for the Tb.N value that differed significantly from that of normal bone (p < 0.05). MAR and BFR/B.Pm presented significantly (p < 0.05) higher values for SF hydrogel-treated defects in comparison with controls treated with a synthetic polymeric material, confirming that SF hydrogel accelerated remodelling processes. (C) 2004 Elsevier Ltd. All rights reserved.