Compound exocytosis in pituitary cells.
Compound exocytosis in pituitary cells.
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垂体细胞中的复合胞吐作用。
DOI：
10.1111/j.1749-6632.2008.04008.x
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发表时间：
2009
影响因子：
5.2
通讯作者：
Zorec,Robert
中科院分区：
文献类型：
--
作者：
Vardjan,Nina;Jorgacevski,Jernej;Stenovec,Matjaz;Kreft,Marko;Zorec,Robert
Neurotransmitter and hormone release from vesicles involves fusion between the vesicle and the plasma membranes, a process termed exocytosis. Recently we reported that most of the spontaneous and stimulated exocytotic events in pituitary lactotrophs are transient and repetitive, appearing in bursts lasting more than 100 s. However, whether this is also the case in compound vesicle‐to‐vesicle exocytosis is unknown. Here we investigated compound exocytotic events in resting and stimulated lactotrophs by using optical and cell‐attached patch‐clamp capacitance measurements. Elementary compound exocytotic events were characterized by multiple‐amplitude on‐steps in synaptopHluorin fluorescence and in membrane capacitance signals. Multiple‐amplitude on‐steps appeared either as a relatively large upward step, indicating that vesicles were fused with each other prior to fusion of the vesicle membrane with the plasma membrane (multivesicular exocytosis), or as a time‐dependent stepwise signal increase, indicating sequential fusion of two or more vesicles with the plasma membrane (sequential exocytosis). In the majority of membrane capacitance recordings (>90%), multiple‐amplitude on‐steps terminated as multiple‐amplitude off‐steps. These complex amplitude events were repetitive, indicating that transient fusion pore openings reflect repetitive interactions of a single vesicle or vesicles in a cluster with the plasma membrane. Out of many mechanisms, these interactions may enable the diffusion of fusion proteins from the plasma membrane to the membrane of the primary fused vesicles, consequently enabling vesicle‐to‐vesicle fusion. The incidence of compound exocytotic events increased by 33% after stimulation, which is consistent with the enhanced efficiency of hormone secretion after the stimulus.
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影响因子：
4.8
作者：
J. Turgeon;D. W. Waring
通讯作者：
J. Turgeon;D. W. Waring
血小板肌球蛋白的纯化和表征。
影响因子：
--
作者：
J. Daniel;J. R. Sellers
通讯作者：
J. R. Sellers
大鼠嗜碱性白血病细胞中抗原诱导的组胺分泌和蛋白激酶 C 磷酸化肌球蛋白。
DOI：
10.1016/s0021-9258(18)63885-2
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ludowyke;I. Peleg;M. Beaven;R. Adelstein
通讯作者：
R. Adelstein
钙和蛋白激酶 C 之间的相互作用在控制垂体促性腺激素的信号传导和分泌中。
影响因子：
4.8
作者：
S. Stojilkovic;T. Iida;F. Merelli;A. Torsello;L. Krsmanović;K. Catt
通讯作者：
K. Catt
影响因子：
4.8
作者：
Paul Thomas;P. Mellon;J. Turgeon;D. W. Waring
通讯作者：
Paul Thomas;P. Mellon;J. Turgeon;D. W. Waring