The intron from the 5′-UTR of the FBP11 gene in petunia displays promoter- and enhancer-like functions
The intron from the 5′-UTR of the FBP11 gene in petunia displays promoter- and enhancer-like functions
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DOI：
10.1016/j.scienta.2013.02.009
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发表时间：
2013-05-02
影响因子：
4.3
通讯作者：
Bao, Manzhu
中科院分区：
文献类型：
--
作者：
Liao, Liao;Ning, Guogui;Bao, Manzhu
现在有证据表明，内含子在许多方面具有重要的生物学功能，包括调节和结构目的，促进遗传多样性，分子进化等。在本研究中，β-葡萄糖醛酸酶基因(GUS)分别位于FBP11启动子(PF11)和5‘-非编码区内含子(F11I)的下游；组织化学染色表明，PF11和F11I都具有激活GUS基因表达的能力。当F11I位于花椰菜花叶病毒(CaMV)35S启动子和GUS基因之间时，GUS基因在转基因烟草中的表达水平比单独的CaMV 35S启动子高3~9倍。这些观察结果表明，矮牵牛花结合蛋白11基因5‘非翻译区的内含子同时具有启动子和增强子的功能。(C)2013爱思唯尔B.V.保留所有权利。
Evidence now exists that introns have many important biological functions in various aspects, including regulation and structural purposes, facilitating genetic diversification, molecular evolution and so on. In this study, the beta-glucuronidase (GUS) gene was separately placed downstream of the FBP11 promoter (PF11) and the intron from the 5 '-UTR (F11I); histochemical staining showed that both the PF11 and F11I have the capacity to activate the expression of the GUS gene. Moreover, when the F11I was placed between the Cauliflower Mosaic Virus (CaMV) 35S promoter and GUS gene, the expression level of the GUS in the transgenic tobacco plants was three to nine fold increases than the CaMV 35S promoter alone. These observations indicate that the intron from the 5'-untranslated region (5'-UTR) of Floral Binding Protein 11 (FBP11) gene in petunia (Petunia hybrida) displays both promoter- and enhancer-like functions. (c) 2013 Elsevier B.V. All rights reserved.