Unusual sequence features and gene rearrangements of primitive crabs revealed by three complete mitochondrial genomes of Dromiacea
Unusual sequence features and gene rearrangements of primitive crabs revealed by three complete mitochondrial genomes of Dromiacea
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德罗米亚科的三个完整线粒体基因组揭示了原始蟹的异常序列特征和基因重排
DOI：
10.1016/j.cbd.2016.07.004
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发表时间：
2016-12-01
影响因子：
3
通讯作者：
Song, Chengwen
中科院分区：
文献类型：
--
作者：
Shi, Guohui;Cui, Zhaoxia;Song, Chengwen
对原始蟹Dynomene pilumnoides（属于Dromioidea）、Homola orientalis 和Moloha Majora（属于Homoloidea）的三个完整线粒体基因组进行了测定、表征，并与其他短蟹类蟹进行了比较。由于存在四个基因间非编码序列（IGN），D.pilumnoides 的完整长度为 16,475 bp，在短链有丝分裂基因组中相对较大。其最长的IGN大小为652 bp，位于trnL1和trnQ之间，考虑到其与同一超科物种Conchoecetes artificiosus和Takedromia cristatipes的高度相似性，被认为是假定的控制区（CR）。与昆虫和甲壳类动物假定祖先的基因顺序相比，D.pilumnoides 有丝分裂基因组表现出三个基因（trnQ、CR 和 trnH）位置易位的重排。 H. orientalis 和 M.majora 的线粒体基因组大小分别为 16,084 bp 和 15,903 bp。它们的基因排列与大多数短尾类物种的报道一致。这两种 Homoloidea 蟹的 CR 长度分别为 1242 bp 和 1037 bp。控制区串联重复序列（TRS）的出现是三种 Homoloidea 蟹（除了 Homologenus malayens）所共有的，但在其他报道的短蟹中没有发现。此外，东方人和马来人的重复单元显示出高水平的序列同一性和稳定的二级结构。系统发育分析表明，Dromoidea 和 Homoloidea 构成 Brachyura 中最基本的组合，其次是 Raninoidea。 (C) 2016 年，爱思唯尔公司出版
Three complete mitochondrial genomes of primitive crabs, Dynomene pilumnoides (belong to Dromioidea), Homola orientalis and Moloha majora (belong to Homoloidea) were determined, characterized and compared with other brachyuran crabs. Due to the presence of four intergenic noncoding sequences (IGNs), the complete length of 16,475 bp in D. pilumnoides is relatively large among brachyuran mitogenomes. Its longest IGN is 652 bp in size locating between trnL1 and trnQ, which is regarded as putative control region (CR) considering its high similarity with the same superfamily species, Conchoecetes artificiosus and Takedromia cristatipes. Compared with the gene order of putative ancestors of insects and crustaceans, D. pilumnoides mitogenome exhibits rearrangements with positional translocation of three genes (trnQ, CR and trnH). The mitogenomes of H. orientalis and M. majora are 16,084 bp and 15,903 bp in size, respectively. Their gene arrangements are consistent with those reported for most brachyuran species. The lengths of CR in these two Homoloidea crabs are 1242 bp and 1037 bp, respectively. The occurrence of tandem repeat sequences (TRS) in control region is shared by the three Homoloidea crabs (besides Homologenus malayens), but is not found in the other reported brachyuran crabs. Moreover, the repeat units in H. orientalis and H. malayens show the high level of sequence identity with stable secondary structure. The phylogenetic analyses indicate that Dromioidea and Homoloidea form the most basal assemblage within Brachyura, followed by Raninoidea. (C) 2016 Published by Elsevier Inc.