Measuring the elastic properties of living cells with atomic force microscopy indentation.
Measuring the elastic properties of living cells with atomic force microscopy indentation.
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DOI：
10.1007/978-1-62703-056-4_15
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Kumar, Sanjay
中科院分区：
文献类型：
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作者：
Mackay, Joanna L;Kumar, Sanjay
原子力显微镜（AFM）是一种功能强大的多功能工具，用于探测生物样品的机械性能。本章描述了使用AFM压痕测量活细胞弹性模量的过程。我们包括使用AFM/光学显微镜系统进行悬臂梁校准和数据采集的分步说明，以及数据分析的详细协议。我们的方案是专门为BioScope Catalyst AFM系统（Bruker AXS Inc.）编写的;不过，大部分的一般概念都可以很容易地转换到其他商业系统。
Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specifically for the BioScope Catalyst AFM system (Bruker AXS Inc.); however, most of the general concepts can be readily translated to other commercial systems.