The Hsp70 heat-shock gene family of the mosquito Anopheles albimanus.
The Hsp70 heat-shock gene family of the mosquito Anopheles albimanus.
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DOI：
10.1111/j.1365-2583.1993.tb00130.x
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发表时间：
1993-01-01
影响因子：
2.6
通讯作者：
Seawright, J.A.
中科院分区：
文献类型：
--
作者：
Benedict, M.Q.;Cockburn, A.F.;Seawright, J.A.
从白纹伊蚊基因组DNA文库中分离到4个白纹伊蚊Hsp70基因，每个克隆含有1对差异转录基因，与果蝇Hsp70基因的蛋白质编码区有75%的DNA序列相似性。通过原位杂交，将克隆定位于2R染色体上的两个基因座。这些克隆对热休克有很强的杂交能力，但与未受刺激的蚊子RNA杂交很弱。白纹伊蚊的Hsp70基因家族正在经历协同进化，可能是通过基因转换。基因的总体排列表明，在两个基因座上不同转录的基因对是一种古老的双翅目排列，早于线虫/环纹夜蛾的分化。
Four Hsp70 genes of the malaria vector Anopheles albimanus were isolated from a genomic DNA library as two non‐overlapping clones each containing a pair of divergently transcribed genes having 75% DNA sequence similarity to the protein‐coding regions of the Drosophila metanogaster Hsp70genes. The clones were assigned to two loci on chromosome 2R by in situ hybridization. These clones hybridize strongly to heat‐shock but only weakly to non‐shocked mosquito RNA. The Hsp70 gene family of A. albimanus is undergoing concerted evolution probably by gene conversion. The general arrangement of the genes suggests that divergently transcribed pairs of genes at two loci is an ancient Dipteran arrangement predating the Nematocera/Cyclorrapha divergence.