Transbilayer complementarity of phospholipids. A look beyond the fluid mosaic model.
Transbilayer complementarity of phospholipids. A look beyond the fluid mosaic model.
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DOI：
10.1021/ja046892a
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发表时间：
2004-08
影响因子：
15
通讯作者：
Jianbing Zhang;Bingwen Jing;N. Tokutake;S. Regen
中科院分区：
文献类型：
--
作者：
Jianbing Zhang;Bingwen Jing;N. Tokutake;S. Regen
通过测量在不可交换的基于DMPE或DSPE的二聚体存在下衍生自1，2-二肉豆蔻酰基-sn-甘油基-3-磷酸乙醇胺（DMPE）和1，2-二硬脂酰基-sn-甘油基-3-磷酸乙醇胺（DSPE）的可交换磷脂单体的最近邻偏好来探测磷脂双层上的脂质-脂质相互作用。这些永久的二聚体促进同型磷脂协会在相同程度上，而相应的不可交换的单体没有影响。这些结果支持一个模型，其中较长的磷脂在一个单层小叶优先与较短的相邻单层。这种跨双层互补性可能在稳定生物膜和促进其两个脂质小叶的组成相互依赖方面发挥重要作用。
Lipid-lipid interactions across a phospholipid bilayer were probed by measuring the nearest-neighbor preferences of exchangeable phospholipid monomers derived from 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) in the presence of nonexchangeable DMPE- or DSPE-based dimers. Each of these permanent dimers promoted homophospholipid association to the same extent, whereas the corresponding nonexchangeable monomers were without effect. These results support a model in which the longer phospholipids in one monolayer leaflet preferentially associate with shorter ones in the adjoining monolayer. Such transbilayer complementarity is likely to play an important role in stabilizing biological membranes and also in promoting a compositional interdependence of their two lipid leaflets.