Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo
Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo
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DOI：
10.1016/j.bmcl.2016.05.076
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发表时间：
2016-08-01
影响因子：
2.7
通讯作者：
Trivedi, Ritu
中科院分区：
文献类型：
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作者：
Kushwaha, Priyanka;Khedgikar, Vikram;Trivedi, Ritu
采用溶剂纯化技术对印楝中的萜类化合物进行了色谱纯化，鉴定为印楝酮(1)、环氧印楝酮(2)印楝酮(3)格杜宁素(4)宁宾素(5)Salannin(6)印楝素A(7)和印楝素B(8)。在8种化合物中，只有3种化合物具有成骨活性，并能促进成骨细胞的增殖、分化和矿化。活性化合物在体外刺激成骨基因ALP、RunX-2和OCN的表达，但与其他两种活性化合物相比，印楝素A刺激成骨细胞分化和矿化的能力最大。在体内研究中，给幼犬皮下注射印楝素A，可显著增强成骨基因表达，促进成骨率。本文认为印楝的活性成分具有成骨活性，印楝素A对骨有有益作用。(C) 2016 Elsevier Ltd.版权所有。
Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone. (C) 2016 Elsevier Ltd. All rights reserved.