Large Scale Parallel DNA Detection by Two-Dimensional Solid-State Multipore Systems
Large Scale Parallel DNA Detection by Two-Dimensional Solid-State Multipore Systems
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DOI：
10.1021/acssensors.8b00192
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发表时间：
2018-05-01
期刊：
影响因子：
8.9
通讯作者：
Leburton, Jean-Pierre
中科院分区：
文献类型：
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作者：
Athreya, Nagendra Bala Murali;Sarathy, Aditya;Leburton, Jean-Pierre
我们描述了一种由纳米级半导体材料制成的密集多个纳米孔阵列的可扩展设备设计，用于在大规模并行检测方案中检测和识别许多生物分子的易位。我们使用分子动力学与纳米级设备模拟相结合来说明该设备设置唯一识别 DNA 平行易位的能力。我们表明，沿膜的横向片电流不受生物分子通过多个孔同时易位所产生的串扰效应的影响，因为它们能够仅感知局部电位变化。我们还表明，与多孔设置中的离子电流阻挡技术相比，跨纳米孔膜的电子传感提供了更高的检测分辨率，而不管在二维膜中制造纳米孔时出现的不规则性。
We describe a scalable device design of a dense array of multiple nanopores made from nanoscale semiconductor materials to detect and identify translocations of many biomolecules in a massively parallel detection scheme. We use molecular dynamics coupled to nanoscale device simulations to illustrate the ability of this device setup to uniquely identify DNA parallel translocations. We show that the transverse sheet currents along membranes are immune to the crosstalk effects arising from simultaneous translocations of biomolecules through multiple pores, due to their ability to sense only the local potential changes. We also show that electronic sensing across the nanopore membrane offers a higher detection resolution compared to ionic current blocking technique in a multipore setup, irrespective of the irregularities that occur while fabricating the nanopores in a two-dimensional membrane.