Antigen processing and CD24 expression determine antigen presentation by splenic CD4+ and CD8+ dendritic cells
Antigen processing and CD24 expression determine antigen presentation by splenic CD4+ and CD8+ dendritic cells
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DOI：
10.1111/j.1365-2567.2007.02711.x
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发表时间：
2008-03-01
期刊：
影响因子：
6.4
通讯作者：
Harding, Clifford V.
中科院分区：
文献类型：
--
作者：
Askew, David;Harding, Clifford V.
为了研究树突状细胞（DC）抗原呈递功能的异质性，我们将小鼠脾树突状细胞分为CD4（+）和CD8（+）两组，并评估其处理和呈递颗粒抗原到CD4（+）和CD8(+) T细胞的能力。CD4（+）和CD8(+) dc都处理外源性颗粒抗原，但CD8(+) dc在主要组织相容性复合体（MHC） II类抗原呈递和MHC I类交叉呈递方面比CD4(+) dc效率更高。虽然抗原处理效率有助于CD8(+) dc的优越抗原递呈功能，但我们的研究也揭示了CD24的重要贡献。CD8(+) dc在诱导幼稚T细胞获得某些效应T细胞功能方面也比CD4(+) dc更有效，例如产生细胞毒性CD8(+) T细胞和产生干扰素(IFN)- γ的CD4(+) T细胞。总之，CD8(+) dc是特别有效的抗原呈递细胞，表达关键的共刺激因子，并有效地处理外源抗原，以供MHC I类和II类分子呈递。
To examine heterogeneity in dendritic cell (DC) antigen presentation function, murine splenic DCs were separated into CD4(+) and CD8(+) populations and assessed for the ability to process and present particulate antigen to CD4(+) and CD8(+) T cells. CD4(+) and CD8(+) DCs both processed exogenous particulate antigen, but CD8(+) DCs were much more efficient than CD4(+) DCs for both major histocompatibility complex (MHC) class II antigen presentation and MHC class I cross-presentation. While antigen processing efficiency contributed to the superior antigen presentation function of CD8(+) DCs, our studies also revealed an important contribution of CD24. CD8(+) DCs were also more efficient than CD4(+) DCs in inducing naive T cells to acquire certain effector T-cell functions, for example generation of cytotoxic CD8(+) T cells and interferon (IFN)-gamma-producing CD4(+) T cells. In summary, CD8(+) DCs are particularly potent antigen-presenting cells that express critical costimulators and efficiently process exogenous antigen for presentation by both MHC class I and II molecules.