Liquid crystal-based aptasensor for the detection of interferon-γ and its application in the diagnosis of tuberculosis using human blood
Liquid crystal-based aptasensor for the detection of interferon-γ and its application in the diagnosis of tuberculosis using human blood
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DOI：
10.1016/j.snb.2018.11.104
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发表时间：
2019-03-01
影响因子：
8.4
通讯作者：
Jang, Chang-Hyun
中科院分区：
文献类型：
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作者：
Kim, Hyeong Jin;Jang, Chang-Hyun
我们报道了一种基于液晶(LC)的适体传感器的发展，用于检测人血液中的干扰素-γ(干扰素-γ)以及结核病(TB)的诊断。在这个系统中，干扰素-γ与固定在表面的适配子的结合破坏了LCS的取向，导致了从同向取向到随机取向的转变。这种液晶取向的变化可以很容易地转换，并在偏振光显微镜下观察到从暗光学LC图像到明亮图像的转变。通过这种传感机制，可以检测到低至1 PM(17pg/ml)的干扰素-γ水平。这种基于LC的适配子传感器被用于利用潜伏性结核病患者的血液样本来诊断结核病。使用这种基于LC的方法，不仅可以很容易地检测到干扰素-伽马，而且可以使用人血简单而有效地诊断潜伏性结核病，而不需要任何复杂的过程或仪器。因此，我们的研究为干扰素-γ传感器在各种感染性疾病的临床诊断和免疫学研究中的应用提供了一种很有前途的传感器。
We report the development of a liquid crystal (LC)-based aptasensor for the detection of interferon-gamma (IFN-gamma) in human blood as well as the diagnosis of tuberculosis (TB). In this system, the binding of IFN-gamma to an aptamer immobilized on a surface disrupts the orientation of LCs, inducing a transition from a homeotropic orientation to a random one. This change in the orientation of the LCs can easily be converted and observed as a shift from a dark optical LC image to a bright one under a polarized light microscope. Through this sensing mechanism, IFN-gamma levels as low as 1 pM (17 pg/ml) could be detected. This LC-based aptasensor was employed for the diagnosis of TB using blood samples from patients with latent TB. With this LC-based approach, not only could IFN-gamma readily be detected, but also latent TB could be diagnosed using human blood simply and effectively without any intricate processes or instrumentation. Therefore, our present research provides a promising IFN-gamma sensor with applications in clinical diagnosis of various infectious diseases, and in immunological research.