CIRCUIT SIMULATION OF GENETIC NETWORKS
CIRCUIT SIMULATION OF GENETIC NETWORKS
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DOI：
10.1126/science.7624793
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发表时间：
1995-08-04
期刊：
影响因子：
56.9
通讯作者：
SHAPIRO, L
中科院分区：
文献类型：
--
作者：
MCADAMS, HH;SHAPIRO, L
用目前可用的技术很难分析包含数十到数百个基因的遗传网络。由于这些基于生物化学的遗传电路和电路在功能上有许多相似之处，因此提出了一种混合建模方法，该方法将传统的生化动力学建模集成在电路仿真的框架内。噬菌体λ裂解-裂解决定电路的电路图表示生化成分的信号路径中的连通性。Lambda遗传电路的一个关键特征是操纵子作为活跃的集成逻辑组件发挥作用，并引入信号时延，这是噬菌体lambda在体内行为所必需的。
Genetic networks with tens to hundreds of genes are difficult to analyze with currently available techniques. Because of the many parallels in the function of these biochemically based genetic circuits and electrical circuits, a hybrid modeling approach is proposed that integrates conventional biochemical kinetic modeling within the framework of a circuit simulation. The circuit diagram of the bacteriophage lambda lysis-lysogeny decision circuit represents connectivity in signal paths of the biochemical components. A key feature of the lambda genetic circuit is that operons function as active integrated logic components and introduce signal time delays essential for the in vivo behavior of phage lambda.