A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile
A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile
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DOI：
10.1016/j.phytochem.2015.11.012
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发表时间：
2016-02-01
期刊：
影响因子：
3.8
通讯作者：
Novak, Ondrej
中科院分区：
文献类型：
--
作者：
Flokova, Kristyna;Feussner, Kirstin;Novak, Ondrej
茉莉酸(Jas)是一种将外界胁迫刺激与生理反应相结合的植物激素。(+)-7-iso-JA-L-ILE是已知JA受体的一种成分，是COI1的天然JA配体。JA的上游合成前体顺-(+)-12-氧代植物二烯酸(cis-(+)-OPDA)被报道在胁迫诱导和发育过程中独立于COI1发挥作用。创伤诱导的内源性JA/JA-Ile水平的增加伴随着OPDA浓度的2~10倍的增加，但其感知和代谢的方式尚不清楚。为了筛选可能的OPDA代谢物，用25%的甲醇水溶液(v/v)提取拟南芥营养组织，采用反相聚合物固相萃取一步法进行纯化，并用高通量质谱仪进行分析。这使得能够在植物组织样品中检测和定量生物活性(+)-7-iso-JA-L-Ile的低丰度OPDA类似物。对新鉴定的化合物及其相关植物激素JA、JA-Ile和顺式(+)-OPDA的水平进行了监测，并与在JA(dde2-2，opr3)和JA-Ile(Jar1)生物合成缺失的拟南芥突变体中观察到的水平进行了比较。观察到的cis-(+)-OPDA-Ile水平差异很大，这引发了人们对其在拟南芥胁迫反应中所起作用的质疑。(C)2015爱思唯尔有限公司。保留所有权利。
Jasmonates (JAs) are plant hormones that integrate external stress stimuli with physiological responses. (+)-7-iso-JA-L-Ile is the natural JA ligand of COI1, a component of a known JA receptor. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-(+)-OPDA) has been reported to act independently of COI1 as an essential signal in several stress-induced and developmental processes. Wound induced increases in the endogenous levels of JA/JA-Ile are accompanied by two to tenfold increases in the concentration of OPDA, but its means of perception and metabolism are unknown. To screen for putative OPDA metabolites, vegetative tissues of flowering Arabidopsis thaliana were extracted with 25% aqueous methanol (v/v), purified by single-step reversed-phase polymer-based solid-phase extraction, and analyzed by high throughput mass spectrometry. This enabled the detection and quantitation of a low abundant OPDA analog of the biologically active (+)-7-iso-JA-L-Ile in plant tissue samples. Levels of the newly identified compound and the related phytohormones JA, JA-Ile and cis-(+)-OPDA were monitored in wounded leaves of flowering Arabidopsis lines (Col-0 and Ws) and compared to the levels observed in Arabidopsis mutants deficient in the biosynthesis of JA (dde2-2, opr3) and JA-Ile (jarl). The observed cis-(+)-OPDA-Ile levels varied widely, raising,questions concerning its role in Arabidopsis stress responses. (C) 2015 Elsevier Ltd. All rights reserved.