Quantitative analysis of docetaxel in human plasma using liquid chromatography coupled with tandem mass spectrometry
Quantitative analysis of docetaxel in human plasma using liquid chromatography coupled with tandem mass spectrometry
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DOI：
10.1002/bmc.457
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发表时间：
2005-06-01
影响因子：
1.8
通讯作者：
Beijnen, JH
中科院分区：
文献类型：
--
作者：
Kuppens, IELM;van Maanen, MJ;Beijnen, JH
描述了一种定量测定人血浆中多西他赛的方法。使用叔丁基乙醚进行液-液萃取，然后使用碱性洗脱液进行高效液相色谱分析，从基质中提取多西紫杉醇。紫杉醇用作内标。正离子电喷雾串联质谱进行选择性和灵敏的检测。根据FDA生物分析方法验证指南对该方法进行了验证。使用200 μ L血浆等分试样，多西他赛的验证范围为0.25-1000 ng/mL。该方法仅需要有限体积（200 μ L）的人血浆，并且该方法可应用于需要0.25 ng/mL定量下限的研究。该方法已成功应用于多项多西他赛临床和药理学研究。版权所有（c）2004年约翰威利父子有限公司。
An assay for the quantitative determination of docetaxel in human plasma is described. Docetaxel was extracted from the matrix using liquid-liquid extraction with ter-butylinethylether, followed by high-performance liquid chromatographic analysis using an alkaline eluent. Paclitaxel was used as internal standard. Positive ionization electrospray tandem mass spectrometry was performed for selective and sensitive detection. The method was validated according to the FDA guidelines on bioanalytical method validation. The validated range for docetaxel was from 0.25-1000 ng/mL using 200 mu L plasma aliquots. The method requires only a limited volume (200 mu L) of human plasma and the method can be applied in studies requiring a low lower limit of quantitation of 0.25 ng/mL. The assay was applied successfully in several clinical and pharmacological studies with docetaxel. Copyright (c) 2004 John Wiley & Sons, Ltd.