Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibility
Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibility
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DOI：
10.1007/s11103-006-0032-9
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发表时间：
2006-07-01
影响因子：
5.1
通讯作者：
Nishio, Takeshi
中科院分区：
文献类型：
--
作者：
Fujimoto, Ryo;Sugimura, Tetsu;Nishio, Takeshi
研究了具有自交亲和I类S单倍型S-f2的芜菁自交亲和品种Yellow Sarson的S位点突变。甘蓝S-28与B的S-f2是一对种间配对。拉帕。B中S-f2的原始S单倍型被鉴定为S-54。拉帕。S-f2与S-54和B等位基因的序列比较。oleracea S-28中SRK的第一个内含子插入了一个逆转录转座子样序列，SP11的启动子区缺失了89 bp。在S-f2纯合子中未检测到SRK和SP11的转录本，表明SRK和SP11的插入和缺失分别导致了这两个基因功能的丧失。转基因植株的启动子检测表明，S-f2的SP11启动子没有活性。B中S-f2和正常II类S单倍型S-60的杂合子。发现芜菁是自交亲和的。有趣的是，在S-f2/S-60杂合子中，SP11-60的转录被抑制，这表明未转录的I类SP11等位基因抑制S杂合子花药中隐性II类SP11等位基因的表达。在B中，一个自交亲和的I类S单倍型和一个自交不亲和的II类S单倍型的杂合子也观察到类似的现象。甘蓝属植物
Mutations in the S locus of a self-compatible cultivar Yellow Sarson in Brassica rapa, which has a self-compatible class-I S haplotype, S-f2, were investigated. S-28 in Brassica oleracea was found to be a member of an interspecific pair with S-f2 in B. rapa. The original S haplotype of S-f2 was identified to be S-54 in B. rapa. Sequence comparison of alleles in S-f2 with those in S-54 and B. oleracea S-28 revealed insertion of a retrotransposon-like sequence in the first intron of SRK and 89-bp deletion in the promoter region of SP11. No transcripts of SRK and SP11 were detected in S-f2 homozygotes, suggesting that the insertion and the deletion in SRK and SP11, respectively, caused the loss of the function of these genes. Promoter assay using transgenic plants indicated that the SP11 promoter of S-f2 has no activity. Heterozygotes of S-f2 and a normal class-II S haplotype, S-60, in B. rapa were found to be self-compatible. Interestingly, transcription of SP11-60 was revealed to be suppressed in the S-f2/S-60 heterozygotes, suggesting that an untranscribed class-I SP11 allele suppresses the expression of a recessive class-II SP11 allele in the anthers of S heterozygotes. Similar phenomenon was observed in heterozygotes of a self-compatible class-I S haplotype and a self-incompatible class-II S haplotype in B. oleracea.