Phytosterol content and the campesterol: sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation
Phytosterol content and the campesterol: sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation
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植物甾醇含量和菜油甾醇：谷甾醇比例影响棉纤维发育：植物甾醇在细胞伸长中的作用
DOI：
10.1007/s11427-015-4992-3
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发表时间：
2016-02-01
影响因子：
9.1
通讯作者：
Luo, Ming
中科院分区：
文献类型：
--
作者：
Deng, Shasha;Wei, Ting;Luo, Ming
植物甾醇在植物生长发育过程中起着重要作用，包括细胞分裂、细胞伸长、胚胎发生、纤维素合成和细胞壁形成。棉纤维是研究植物细胞伸长和细胞壁发生的理想模型，其细胞伸长和纤维素合成同步进行。通过用甾醇生物合成抑制剂十三吗啉处理纤维，研究了植物甾醇在纤维生长中的作用。植物甾醇生物合成的抑制导致纤维伸长在体外或在植物中的明显抑制。植物甾醇含量的测定表明，谷甾醇和菜油甾醇是棉纤维中主要的植物甾醇，且在纤维快速伸长期间含量较高。此外，菜油甾醇：谷甾醇比率的降低和增加分别与伸长速度的增加和减少有关。该比率的增加与从细胞伸长到次生细胞壁合成的过渡有关。此外，一些植物甾醇生物合成基因下调ligon lintless-1突变体的短纤维，相比其近等基因野生型TM-1。这些结果表明，植物甾醇在棉纤维发育，特别是在纤维伸长中起着至关重要的作用。
Phytosterols play an important role in plant growth and development, including cell division, cell elongation, embryogenesis, cellulose biosynthesis, and cell wall formation. Cotton fiber, which undergoes synchronous cell elongation and a large amount of cellulose synthesis, is an ideal model for the study of plant cell elongation and cell wall biogenesis. The role of phytosterols in fiber growth was investigated by treating the fibers with tridemorph, a sterol biosynthetic inhibitor. The inhibition of phytosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta. The determination of phytosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers; moreover, higher concentrations of these phytosterols were observed during the period of rapid elongation of fibers. Furthermore, the decrease and increase in campesterol:sitosterol ratio was associated with the increase and decease in speed of elongation, respectively, during the elongation stage. The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis. In addition, a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1 mutant, compared to its near-isogenic wild-type TM-1. These results demonstrated that phytosterols play a crucial role in cotton fiber development, and particularly in fiber elongation.