Characteristic interactions with phosphatidylinositol 4,5-bisphosphate determine regulation of Kir channels by diverse modulators
Characteristic interactions with phosphatidylinositol 4,5-bisphosphate determine regulation of Kir channels by diverse modulators
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DOI：
10.1074/jbc.m403413200
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发表时间：
2004-09-03
影响因子：
4.8
通讯作者：
Logothetis, DE
中科院分区：
文献类型：
--
作者：
Du, XO;Zhang, HL;Logothetis, DE
特异性内向整流钾（Kir）通道的活性由许多不同调节剂中的任一种调节，例如蛋白激酶C、G（q）偶联受体刺激、pH、细胞内Mg 2+或G蛋白的β-γ亚基。磷脂酰肌醇4，5-二磷酸（PIP 2）是维持所有Kir通道活性的重要因子。在这里，我们证明了通道-PIP 2相互作用的强度决定了Kir通道对各种调节剂调节的敏感性。此外，我们的研究结果表明，Kir通道之间的差异，在其特定的调节，由一个给定的调制器可能反映了其表观亲和力的差异与PIP 2的相互作用。
The activity of specific inwardly rectifying potassium (Kir) channels is regulated by any of a number of different modulators, such as protein kinase C, G(q)-coupled receptor stimulation, pH, intracellular Mg2+ or the betagamma-subunits of G proteins. Phosphatidylinositol 4,5-bisphosphate (PIP2) is an essential factor for maintenance of the activity of all Kir channels. Here, we demonstrate that the strength of channel-PIP2 interactions determines the sensitivity of Kir channels to regulation by the various modulators. Furthermore, our results suggest that differences among Kir channels in their specific regulation by a given modulator may reflect differences in their apparent affinity of interactions with PIP2.