The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro
The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro
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DOI：
10.1002/adbi.201800012
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发表时间：
2018-06-01
影响因子：
4.1
通讯作者：
Cusack, Maggie
中科院分区：
文献类型：
--
作者：
Alakpa, Enateri V.;Saeed, Anwer;Cusack, Maggie
双壳类软体动物大珠蚌的壳由碳酸钙多晶体方解石和文石（珍珠石）组成。珍珠母贝在体内具有诱导脊椎动物细胞成骨的作用，具有良好的骨整合特性。然而，方解石对应物在脊椎动物细胞的反应方面的研究较少。本研究表明，方解石表面形貌与内在化学成分的分离可以实现骨髓间充质干细胞（MSCs）的长期培养。自我更新从自我更新标记CD63、CD166和CD271基因表达的增加可以看出，这表明在方解石地形上培养的细胞保持了其干细胞表型。骨髓间充质干细胞也能保持其多能性，并能成功分化为成骨细胞和脂肪细胞。当指向脂肪形成时，在棱柱复制品上培养的间充质干细胞比在组织培养聚苯乙烯上培养的间充质干细胞更容易分化，这表明在方解石P. maxima棱柱形地形上生长的间充质干细胞具有更高的可塑性。该研究强调了P. maxima方解石地形作为一种支持MSC群体体外扩增的仿生设计的潜力，如果它满足用于治疗用途的自体MSC的需求，这是至关重要的。
The shell of the bivalve mollusc Pinctada maxima is composed of the calcium carbonate polymorphs calcite and aragonite (nacre). Mother-of-pearl, or nacre, induces vertebrate cells to undergo osteogenesis and has good osteointegrative qualities in vivo. The calcite counterpart, however, is less researched in terms of the response of vertebrate cells. This study shows that isolation of calcite surface topography from the inherent chemistry allows viable long-term culture of bone marrow derived mesenchymal stem cells (MSCs). Self-renewal is evident from the increased gene expression of the self-renewal markers CD63, CD166, and CD271 indicating that cells cultured on the calcite topography maintain their stem cell phenotype. MSCs also retain their multipotency and can undergo successful differentiation into osteoblasts and adipocytes. When directed to adipogenesis, MSCs cultured on prism replicas are more amenable to differentiation than MSCs cultured on tissue culture polystyrene indicating a higher degree of plasticity in MSCs growing on calcite P. maxima prismatic topography. The study highlights the potential of the calcite topography of P. maxima as a biomimetic design for supporting expansion of MSC populations in vitro, which is of fundamental importance if it meets the demands for autologous MSCs for therapeutic use.