Infection rate of Ixodes ricinus ticks with Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto in Slovenia.
Infection rate of Ixodes ricinus ticks with Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto in Slovenia.
复制标题
斯洛文尼亚蓖麻硬蜱对阿氏疏螺旋体、加氏疏螺旋体和严格伯氏疏螺旋体的感染率。
DOI：
10.1007/bf01691382
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Picken,RN
中科院分区：
文献类型：
--
作者：
Strle,F;Cheng,Y;Nelson,JA;Picken,MM;Bouseman,JK;Picken,RN
1993年春季，从斯洛文尼亚的六个地区收集了蓖麻伊蚊，以确定它们感染伯氏疏螺旋体的总体比率，并评估这些蜱种群中单个物种的频率。解剖蜱，将中肠组织接种到改良的barbor - stoenner - kelly （BSK II）培养基中。利用种特异性聚合酶链反应（PCR）引物和大限制性片段模式（LRFP）分析将疏螺旋体分离物区分为不同的种。在调查的所有六个地区都发现了受感染的蜱虫。363只蜱中69只（19%）分离到螺旋体，其中成年雌蜱分离率为35%(23/66)，成年雄蜱分离率为22%(20/91)，雌雄蜱分离率为13%（26/206）。对60株分离株的种类测定显示，阿氏疏螺旋体32株（53%），加里氏疏螺旋体20株（33%），严格伯氏疏螺旋体8株（13%）。在卢布尔雅那地区，阿兹伯氏疏螺旋体和加里伯氏疏螺旋体占多数（分别为43%和40%），而感性伯氏疏螺旋体仅占分离株的17%。在该国的其他三个地区，虽然调查的分离株数量很少，但已完全分离出阿兹利螺旋体。本研究表明，在斯洛文尼亚蜱虫种群中，以及在小于100平方米的地理区域内，存在所有三种欧洲勃氏疏螺旋体。
In spring 1993,Ixodes ricinusticks were collected from six regions of Slovenia to determine their overall rate of infection withBorrelia burgdorferisensu lato and to assess the frequency of individual species in these tick populations. Ticks were dissected and midgut tissue inoculated into modified Barbour-Stoenner-Kelly (BSK II) medium.Borreliaisolates were differentiated into separate species using species-specific polymerase chain reaction (PCR) primers and by large restriction fragment pattern (LRFP) analysis. Infected ticks were found in all six regions surveyed. Spirochaetes were isolated from 69 of 363 ticks (19 %): the isolation rate from adult female ticks was 35 % (23/66 ticks cultured), from adult male ticks 22 % (20/91), and from nymphal ticks 13 % (26/206). Determination of the species of 60 isolates revealed that 32 wereBorrelia afzelii(53 %), 20 wereBorrelia garinii(33 %), and 8 wereBorrelia burgdorferisensu stricto (13 %). In the Ljubljana regionBorrelia afzeliiandBorrelia gariniipredominated (43 % and 40 %, respectively), whereasBorrelia burgdorferi sensustricto constituted only 17 % of isolates. In three other regions of the countryBorrelia afzeliiwasisolated exclusively, although the number of isolates investigated was small. This study demonstrates the presence of all three European species ofBorrelia burgdorferi sensulato within the Slovenian tick population and also within a geographic area of less than 100 m2.