IMPACT OF LOW-TEMPERATURE STRESS ON GENERAL PHENYLPROPANOID AND ANTHOCYANIN PATHWAYS - ENHANCEMENT OF TRANSCRIPT ABUNDANCE AND ANTHOCYANIN PIGMENTATION IN MAIZE SEEDLINGS
IMPACT OF LOW-TEMPERATURE STRESS ON GENERAL PHENYLPROPANOID AND ANTHOCYANIN PATHWAYS - ENHANCEMENT OF TRANSCRIPT ABUNDANCE AND ANTHOCYANIN PIGMENTATION IN MAIZE SEEDLINGS
复制标题
DOI：
10.1007/bf00714468
复制
发表时间：
1994-09-01
期刊：
影响因子：
4.3
通讯作者：
WALBOT, V
中科院分区：
文献类型：
--
作者：
CHRISTIE, PJ;ALFENITO, MR;WALBOT, V
在玉米（Zea mays L.）中，花青苷含量和其产物在一般苯丙烷代谢和花青苷途径中起作用的基因的转录丰度的变化被监测。短期低温处理的幼苗。玉米幼苗叶鞘中花青苷和mRNA的丰度随低温强度和持续时间的增加而增加。花青素积累被发现在所有的测试线，是基因型的任何花青素生产能力。在将7-d玉米（B37 N）幼苗转移到10 ℃的24小时内，苯丙氨酸解氨酶（Phe）（EC 4.3.1.5）-同源和查耳酮合酶（C2）（EC 2.3.1.74）转录物水平分别增加至少8倍和50倍，并且4-香豆酸：CoA连接酶（4Cl）（EC 6.2.1.12）-同源和查耳酮异构酶（Chi）（EC 5.5.1.6）-同源转录物增加至少3倍于未胁迫植物中的水平。时程研究表明，C2（EC 4.3.1.5）和C2-转录水平保持相对恒定的第一个12小时的冷胁迫，显着增加，在接下来的12小时，并下降到预处理水平内返回冷胁迫幼苗到环境温度（25摄氏度）的2天。转录本4Cl（EC 6.2.1.12）和Chi（EC 5.5.1.6）在冷胁迫的6小时内大量增加，在接下来的36小时内没有进一步增加，并且在将幼苗返回到25 ℃时下降到预处理水平。转录同源的两个监管（R，C1）和三个结构（A1，A2和Bz 2）花青素基因增加了至少7至10倍，在冷处理，表现出类似的积累动力学为ESTO（EC 4.3.1.5）和C2转录。由Bz 1编码的转录本，花色素苷结构基因UDP：葡萄糖-黄酮醇葡糖基转移酶（EC 2.4.1.91），在对照组织中相对丰富，在寒冷时期仅表现出短暂的增加。我们的研究表明，花色素苷生物合成途径的基因可以被认为是cor（冷调节）基因，并且由于该途径是明确定义的，它是表征植物分子对低温反应的极好主题。
Changes in anthocyanin content and transcript abundance for genes whose products function in general phenylpropanoid metabolism and the anthocyanin pathway were monitored in maize (Zea mays L.) seedlings during short-term, low-temperature treatment. Anthocyanin and mRNA abundance in sheaths of maize seedlings increased with the severity and duration of cold. Anthocyanin accumulation was found in all tested lines that were genotypically capable of any anthocyanin production. Within 24 h of transferring 7-d maize (B37N) seedlings to 10 degrees C, phenylalanine ammonia-lyase (Pal) (EC 4.3.1.5)-homologous and chalcone synthase (C2) (EC 2.3.1.74) transcript levels increased at least 8- and 50-fold, respectively, and 4-coumarate:CoA ligase (4Cl) (EC 6.2.1.12)-homologous and chalcone isomerase (Chi) (EC 5.5.1.6)-homologous transcripts increased at least 3-fold over levels in unstressed plants. Time-course studies showed that Pal (EC 4.3.1.5) and C2-transcript levels remained relatively constant for the first 12 h of cold stress, dramatically increased over the next 12 h, and declined to pretreatment levels within 2 d of returning cold-stressed seedlings to ambient (25 degrees C) temperature. Transcripts 4Cl (EC 6.2.1.12) and Chi (EC 5.5.1.6) increased in abundance within 6 h of cold stress, exhibited no further increase over the next 36 h, and declined to pretreatment levels upon returning seedlings to 25 degrees C. Transcripts homologous to two regulatory (R, C1) and three structural (A1, A2, and Bz2) anthocyanin genes increased at least 7- to 10-fold during cold treatment, exhibiting similar kinetics of accumulation as for Pal (EC 4.3.1.5) and C2 transcripts. Transcripts encoded by Bz1, the anthocyanin structural gene for UDP:glucose-flavonol glucosyltransferase (EC 2.4.1.91), were relatively abundant in control tissues and exhibited only a transient increase during the cold period. Our studies suggest that the genes of the anthocyanin biosynthetic pathway can be considered cor (Cold-Regulation) genes, and because this pathway is well defined, it is an excellent subject for characterizing plant molecular responses to low temperatures.