PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE 5HT3 RECEPTOR, A SEROTONIN-GATED ION CHANNEL
PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE 5HT3 RECEPTOR, A SEROTONIN-GATED ION CHANNEL
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DOI：
10.1126/science.1718042
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发表时间：
1991-10-18
期刊：
影响因子：
56.9
通讯作者：
JULIUS, D
中科院分区：
文献类型：
--
作者：
MARICQ, AV;PETERSON, AS;JULIUS, D
神经递质5-羟色胺（5-HT）激活多种第二信使信号系统，并通过它们间接调节离子通道的功能。血清素也直接激活离子通道，这表明它也可以介导快速的兴奋性反应。一个互补的DNA克隆包含这些快速响应通道，5-羟色胺受体的5-HT 3亚型的编码序列，已被分离通过筛选神经母细胞瘤表达库的功能表达的爪蟾卵母细胞中的降钙素门控电流。预测的蛋白质产物具有配体门控离子通道家族的其他成员所共有的许多特征。克隆受体的药理学和电生理学特征与天然5 HT 3受体的性质基本一致。编码这种受体的信使RNA存在于大脑、脊髓和心脏中。该受体定义了一类新的兴奋性配体门控通道。
The neurotransmitter serotonin (5HT) activates a variety of second messenger signaling systems and through them indirectly regulates the function of ion channels. Serotonin also activates ion channels directly, suggesting that it may also mediate rapid, excitatory responses. A complementary DNA clone containing the coding sequence of one of these rapidly responding channels, a 5HT3 subtype of the serotonin receptor, has been isolated by screening a neuroblastoma expression library for functional expression of serotonin-gated currents in Xenopus oocytes. The predicted protein product has many of the features shared by other members of the ligand-gated ion channel family. The pharmacological and electrophysiological characteristics of the cloned receptor are largely consistent with the properties of native 5HT3 receptors. Messenger RNA encoding this receptor is found in the brain, spinal cord, and heart. This receptor defines a new class of excitatory ligand-gated channels.