Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.
Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.
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固相酶免疫分析法检测乙型肝炎表面抗原。
DOI：
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发表时间：
1976
影响因子：
3.4
通讯作者：
A. Schuurs
中科院分区：
文献类型：
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作者：
G. Wolters;L. Kuijpers;J. Kacaki;A. Schuurs
本文报道了固相酶免疫分析法(EIA)检测乙肝表面抗原(HBs)的初步结果。将该方法与四个国家参考小组(荷兰红十字会输血服务中心实验室实验室小组，美国BOB第2和3号参考小组，以及西德哥廷根大学卫生研究所国家病毒性肝炎参考中心第一小组)稀释系列的四份乙肝表面抗原阳性血清的固相放射免疫分析(RIA)进行了比较。此外，对14例急性乙型病毒性肝炎患者进行了每周(长达16周)的随访，比较了两种检测方法的敏感性和特异性。通过肉眼阅读EIA检测结果和比色法检测结果，该检测方法的敏感性和特异性均优于RIA。EIA对ad亚型的敏感性可能略低于RIA，而对ay亚型的敏感性可能略高于RIA。这些微小的敏感性差异可能是由于所用抗血清的特异性。
The preliminary results of a solid-phase enzyme-immunoassay (EIA) for the detection of hepatitis B surface antigen (HBsAg) are presented. This method has been compared with the solid-phase radioimmunoassay (RIA) for HBsAg in dilution series of four HBsAg positive sera four national reference panels (The Laboratory Panel of the Central Laboratory of the Blood Transfusion Service of the Netherlands Red Cross, USA BOB Reference Panels Nos 2 and 3, and the 1st Panel of the National Reference Centre for virus Hepatitis at the Institute of Hygiene of the University of Göttingen, West Germany). In addition, the two test methods were compared in a weekly (up to 16 weeks) follow-up of 14 patients with acute viral hepatitis B. It was seen that, both by reading EIA test results with the naked eye and by colorimetric reading, the sensitivity and specificity of this test method compared very favourably with those of the RIA. EIA may have a slightly lower sensitivity than RIA for the subtype ad, while its sensitivity for the subtype ay may be slightly higher than that of RIA. These minor sensitivity differences may be due to the specificity profiles of the antisera used.