The Amiloride-Sensitive Epithelial Na+ Channel PPK28 Is Essential for Drosophila Gustatory Water Reception
The Amiloride-Sensitive Epithelial Na+ Channel PPK28 Is Essential for Drosophila Gustatory Water Reception
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DOI：
10.1523/jneurosci.0627-10.2010
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发表时间：
2010-05-05
影响因子：
5.3
通讯作者：
Wang, Zuoren
中科院分区：
文献类型：
--
作者：
Chen, Zijing;Wang, Qingxiu;Wang, Zuoren
水感是果蝇特有的味觉形态。水诱导的低渗透压激活特定的味觉受体神经元，然而，在这些神经元中的假定渗透压传感器的分子身份仍然未知。我们发现阿米洛利及其类似物特异性地拮抗水味觉受体神经元的反应和果蝇对水刺激的行为。删除编码阿米洛利敏感性PPK 28通道（DEG/eNaC（变性蛋白/上皮钠通道）家族成员）的基因，消除了水味觉受体神经元的水诱导活性，并大大降低了苍蝇对水的行为反应。PPK 28通道的异位表达的苦味细胞内的中间型感器，使这些感器响应水的刺激。因此，阿米洛利敏感的PPK 28通道可能作为渗透压传感器味觉水的接收在果蝇。
Water sensation is a specific taste modality in the fruit fly. Water-induced hypoosmolarity activates specific gustatory receptor neurons; however, the molecular identity of the putative osmolarity sensor in these neurons remains unknown. We found that amiloride and its analogs specifically antagonized the response of water gustatory receptor neurons and the behavior of flies toward water stimulation. Deletion of the gene that encodes the amiloride-sensitive PPK28 channel, a DEG/eNaC (degenerin/epithelial sodium channel) family member, abolished the water-induced activity of water gustatory receptor neurons and greatly diminished the behavioral response of flies to water. Ectopic expression of the PPK28 channel in the bitter cells within the intermediate-type sensilla renders these sensilla responsive to water stimuli. Thus, the amiloride-sensitive PPK28 channel may serve as the osmolarity sensor for gustatory water reception in the fruit fly.