Ten polymorphic dinucleotide microsatellite markers of the noble scallop Chlamys nobilis
Ten polymorphic dinucleotide microsatellite markers of the noble scallop Chlamys nobilis
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DOI：
10.1111/j.1471-8286.2006.01420.x
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发表时间：
2006-12
期刊：
影响因子：
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通讯作者：
M. Hui;Z. Bao;Aibin Zhan;Xiaoli Hu;W. Lu;D. Chang;Jingjie Hu
中科院分区：
文献类型：
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作者：
M. Hui;Z. Bao;Aibin Zhan;Xiaoli Hu;W. Lu;D. Chang;Jingjie Hu
以贵族扇贝（Chlamys nobilis）为研究对象，设计了22颗富集（CA）n和（GA）n基因组文库的引物。10对引物提供了清晰的多态扩增产物。根据对48个个体的特征分析，等位基因的数量在3 ~ 6个之间。观察杂合度和期望杂合度的变化范围分别为0 ~ 0.88和0.29 ~ 0.76。基因座对间无明显连锁不平衡，10个基因座中有6个符合Hardy-Weinberg平衡。因此，这些标记可能对研究该物种的种群结构有用。
The primers flanking 22 microsatellites isolated from a genomic library enriched for (CA)n and (GA)n were designed in the noble scallop Chlamys nobilis. Ten primer pairs provided clear and polymorphic amplification products. Based on characterization with 48 individuals, the number of alleles ranged from three to six. The values of observed heterozygosity and expected heterozygosity varied from 0 to 0.88 and from 0.29 to 0.76, respectively. No significant linkage disequilibrium between pairs of loci was found and six of 10 loci conformed to the Hardy–Weinberg equilibrium. These markers are therefore potentially useful for studies of the population structure of the species.