Propionate and butyrate induce gene expression of monocarboxylate transporter 4 and cluster of differentiation 147 in cultured rumen epithelial cells derived from preweaning dairy calves1
Propionate and butyrate induce gene expression of monocarboxylate transporter 4 and cluster of differentiation 147 in cultured rumen epithelial cells derived from preweaning dairy calves1
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丙酸和丁酸诱导断奶前牛犊培养的瘤胃上皮细胞中单羧酸转运蛋白 4 和分化簇 147 的基因表达1
DOI：
10.1093/jas/sky334
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发表时间：
2018
影响因子：
3.3
通讯作者：
Matsuyama Shuichi
中科院分区：
文献类型：
--
作者：
Nakamura Sho;Haga Satoshi;Kimura Koji;Matsuyama Shuichi
短链脂肪酸（SCFA）是断奶后反刍动物的主要能量来源。单羧酸转运蛋白 MCT1 和 MCT4 被认为有助于断奶后瘤胃表面 SCFA 的吸收。本研究测量了安乐死后雄性断奶前（22至34日龄，n = 6）和断奶后（55至58日龄，n = 8）犊牛瘤胃上皮细胞中MCT1和MCT4表达的变化，并试图检查SCFA是否刺激这些转运蛋白的表达。在本研究中，还研究了瘤胃中分化簇 147 (CD147) 基因的表达，因为 CD147 被认为是 MCT1 和 MCT4 表达其正确功能的辅助蛋白。断奶后犊牛瘤胃中 MCT1、MCT4 和 CD147 的基因表达水平显着高于断奶前犊牛。在断奶后瘤胃上皮的基底层 (SB) 和棘层 (SS) 中均检测到强 MCT1 免疫反应性。断奶前犊牛中 MCT1 的表达定位于 SB 和 SS 的特定区域。在断奶后犊牛瘤胃上皮的角质层 (SC) 中检测到 MCT4 免疫阳性细胞。然而，在断奶前动物的 SC 中仅检测到低水平的信号。此外，在体外实验中，瘤胃上皮细胞分别与乙酸盐（0.04、0.4和4 mM）、丙酸盐（0.2、2和20 mM）、丁酸盐（0.1、1和10 mM）或β-羟基丁酸盐（BHBA；0.1、1和10 mM）孵育24小时。丙酸和丁酸均诱导 MCT4 和 CD147 基因表达水平增加，但不影响 MCT1 基因表达。醋酸盐和 BHBA 处理对这些基因表达没有显着影响。综上所述，这些结果表明断奶后犊牛瘤胃上皮中 MCT4 和 CD147 基因表达的增加可能是由于来自固体饮食的丙酸盐和丁酸盐的影响，这可能有助于断奶后瘤胃的发育。
Short-chain fatty acids (SCFAs) are the main source of energy for postweaning ruminants. The monocarboxylic acid transporters, MCT1 and MCT4, are thought to contribute to the absorption of SCFAs from the surface of the rumen following weaning. The present study measured changes in MCT1 and MCT4 expression in ruminal epithelial cells isolated from male preweaning (22 to 34 d old,n= 6) and postweaning (55 to 58 d old,n= 8) calves after euthanasia and sought to examine whether SCFAs stimulate the expression of these transporters. In the current study, cluster of differentiation 147 (CD147) gene expression in the rumen was also investigated since CD147 has been considered to act as ancillary protein for MCT1 and MCT4 to express their correct function. The gene expression levels of MCT1, MCT4, and CD147 in the rumen were found to be significantly higher in postweaning calves than in preweaning calves. Strong MCT1 immunoreactivity was detected in both the stratum basale (SB) and the stratum spinosum (SS) in postweaning ruminal epithelium. Expression of MCT1 in preweaning calves was localized to a specific region of the SB and of the SS. MCT4-immunopositive cells were detected in the stratum corneum (SC) of the ruminal epithelium in postweaning calves. However, only a low level of signal was detected in the SC of preweaning animals. Furthermore, in vitro experiments, ruminal epithelial cells were incubated for 24 h with acetate (0.04, 0.4, and 4 mM), propionate (0.2, 2, and 20 mM), butyrate (0.1, 1, and 10 mM), or β-hydroxybutyrate (BHBA; 0.1, 1, and 10 mM), respectively. Both propionate and butyrate induced an increase in the gene expression levels of MCT4 and CD147, but did not affect MCT1 gene expression. There are no significant effects of acetate and BHBA treatment on these gene expressions. Taken together, these results suggest that an increase in MCT4 and CD147 gene expression in the ruminal epithelium of postweaning calves is likely to be due to the effects of propionate and butyrate derived from a solid-based diet, which may contribute to ruminal development following weaning.