Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT.
Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT.
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蛇床子素通过调节 PI3K/AKT 抑制胃癌细胞增殖
DOI：
10.1371/journal.pone.0193449
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Xu X;Liu X;Zhang Y
蛇床子素是从中药蛇床子Cnidium monnieri（L.）Cusson等，并有报道具有抗肿瘤作用。然而，蛇床子素对胃癌细胞的作用尚未见报道。本实验研究蛇床子素对人胃癌细胞增殖的影响。结果表明，蛇床子素能显著抑制胃癌细胞的增殖，并使细胞周期阻滞于G2/M期，且呈剂量依赖性。Western-blot结果显示，蛇床子素可明显抑制细胞周期蛋白B1和cdc 2的表达。PI 3 K和pAKT的表达也被显著抑制，并且结果表明PI 3 K抑制剂显著增强蛇床子素对pAKT、cyclin B1和cdc 2水平的抑制。以上结果表明蛇床子素可通过降低PI 3 K/AKT诱导胃癌细胞周期阻滞于G2/M期而抑制胃癌细胞增殖。
Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric cancer cells were tested. The data showed that osthole treatment significantly inhibited the proliferation of gastric cancer cells and resulted in the cell cycle arrest at G2/M phase in a dose-dependent manner. Western-blot study showed that the expression of cyclin B1 and cdc2 was markedly reduced by osthole. Moreover, expression of PI3K and pAKT was also significantly suppressed, and the results indicated that the inhibition of pAKT, cyclin B1, and cdc2 levels by osthole was notably enhanced by a PI3K inhibitor. These results demonstrate that osthole could inhibit gastric cancer cells proliferation via induction of cell cycle arrest at G2/M phase by the reduction of PI3K/AKT.
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