KINETICS OF REVERSIBLE PROTEIN DENATURATION - STUDY ON APLYSIA MYOGLOBIN
KINETICS OF REVERSIBLE PROTEIN DENATURATION - STUDY ON APLYSIA MYOGLOBIN
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DOI：
10.1016/0301-4622(79)80012-5
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发表时间：
1979-01-01
影响因子：
3.8
通讯作者：
BRUNORI, M
中科院分区：
文献类型：
--
作者：
GIACOMETTI, GM;ANTONINI, E;BRUNORI, M
本文报道了用温度突变和停流法对南极虾肌红蛋白进行变性和复性的动力学研究。展开和重折叠的时间过程揭示了在不存在和存在醇的情况下存在大量的中间体。提出并讨论了线性三态反应机理。
The kinetics of denaturation and renaturation of Aplysia myoglobin by temperature-jump and stopped-flow are reported. The time course of the unfolding and refolding reveals the presence of significant amounts of intermediates both in the absence and in the presence of alcohols. A linear 3-states reaction mechanism is proposed and discussed.