Interleukin 6 is a differentiation factor for human megakaryocytes in vitro.
Interleukin 6 is a differentiation factor for human megakaryocytes in vitro.
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Interleukin 6 是人巨核细胞的体外分化因子。
DOI：
10.1002/eji.1830200909
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发表时间：
1990
影响因子：
5.4
通讯作者：
Burstein,SA
中科院分区：
文献类型：
--
作者：
Kimura,H;Ishibashi,T;Uchida,T;Maruyama,Y;Friese,P;Burstein,SA
通过将白细胞介素6（IL 6）加入人骨髓（BM）培养物中，研究了巨核细胞谱系细胞对IL 6（一种具有多种生物活性的细胞因子）的反应。单独的IL 6对甲基纤维素中巨核细胞集落形成没有影响;然而，在最大刺激浓度的IL 3存在下，观察到集落形成几乎增加了两倍。用生长因子孵育2 h的BM的氚化胸苷自杀研究显示，用IL 3或IL 3 + IL 6预孵育的几乎一半的巨核细胞祖细胞（CFU-Mk）处于S期，而单独用IL 6孵育的BM与对照相似（约24%的CFU-Mk处于S期）。当向BM的液体悬浮培养物中加入0.1ng/ml的IL 6时，与对照培养物中观察到的相比，单个巨核细胞的大小显著增加。此外，与用IL 3生长的培养物（模态倍性8 N）相比，在IL 6存在下生长的巨核细胞的DNA含量增加（模态倍性16 N）。为了确定IL 6的作用是否是直接的而不是通过辅助BM细胞介导的，将该因子加入分离的单个巨核细胞的培养物中。在IL 6存在的情况下，71%的细胞直径增加，而在不存在该因子的情况下，只有27%的细胞大小增加。数据显示IL 6是人巨核细胞的直接成熟因子，促进这些细胞的大小和倍性的增加。
The response of cells of the megakaryocytic lineage to interleukin 6 (IL 6), a cytokine with multiple biological activities, was studied by adding the factor to human bone marrow (BM) cultures. IL 6 alone had no effect on megakaryocytic colony formation in methylcellulose; however, in the presence of maximally stimulating concentrations of IL 3, almost a twofold increment in colony formation was observed. Tritiated thymidine suicide studies of BM incubated for 2 h with growth factors showed that almost one‐half of megakaryocytic progenitors (CFU‐Mk) preincubated with IL3 or IL3 plus IL6 were in S phase, whereas BM incubated with IL 6 alone was similar to control (∽ 24% of CFU‐Mk in S phase). When ≧ 1 ng/ml of IL6 was added to liquid suspension cultures of BM, the size of individual megakaryocytes was significantly augmented compared with that seen in control cultures. Moreover, the DNA content of megakaryocytes grown in the presence of IL6 was increased (modal ploidy 16N) compared with cultures grown with IL3 (modal ploidy 8N). To determine if the effect of IL6 could be direct rather than mediated via accessory BM cells, the factor was added to cultures of isolated single megakaryocytes. Seventy‐one percent of cells increased in diameter in the presence of IL6, while only 27% increased in size in the absence of the factor. The data show that IL6 is a direct maturation factor for human megakaryocytes, promoting increments in size and ploidy of these cells.