Proteomic characterization of Pseudomonas aeruginosa PAO1 inner membrane
Proteomic characterization of Pseudomonas aeruginosa PAO1 inner membrane
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DOI：
10.1002/pmic.201200565
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发表时间：
2013-08-01
期刊：
影响因子：
3.4
通讯作者：
Coute, Yohann
中科院分区：
文献类型：
--
作者：
Casabona, Maria G.;Vandenbrouck, Yves;Coute, Yohann
铜绿假单胞菌是一种革兰氏阴性细菌，可以栖息在各种环境中并感染不同的宿主。人类感染这种微生物现在被认为是一个主要的健康问题，特别是由于其多重耐药性。本数据集提供了铜绿假单胞菌内膜蛋白质组的第一个描述。为了实现这一点，我们结合了有效的分离膜从PAO1参考菌株使用不连续蔗糖梯度离心和MS为基础的蛋白质组学分析。建立了991个非冗余蛋白质的核心列表，并在跨膜结构域、信号肽和脂盒序列预测方面进行了分析。此外，跨膜和膜相关蛋白质复合物的功能的见解进行了探索。所有质谱数据均已保存在ProteomeXchange中，标识符为PXD 000107。
Pseudomonas aeruginosa is a Gram-negative bacterium that can inhabit a wide variety of environments and infect different hosts. Human infections by this microbe are nowadays perceived as a major health problem due notably to its multiresistance. The present data set provides the first description of P. aeruginosa inner membrane proteome. To achieve this, we combined efficient separation of membranes from PAO1 reference strain using discontinuous sucrose gradient centrifugation and MS-based proteomic analysis. A core list of 991 nonredundant proteins was established and analyzed in terms of trans-membrane domains, signal peptide, and lipobox sequence prediction. Furthermore, functional insights into membrane-spanning and membrane-associated protein complexes have been explored. All mass spectrometry data have been deposited in the ProteomeXchange with identifier PXD000107.