Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells
Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells
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DOI：
10.1136/bmjophth-2018-000217
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发表时间：
2019-01-01
影响因子：
2.4
通讯作者：
Negishi, Kazuno
中科院分区：
文献类型：
--
作者：
Niwano, Yoshimi;Iwasawa, Atsuo;Negishi, Kazuno
目的蓝光对视网膜和眼表的危害已被反复描述，并介绍了多种视网膜防护方法，但尚未有关于眼表防护的研究。本实验的目的是检测蓝光照射对原代人角膜表面细胞的光毒性和遮荫保护作用。方法从眼库中获取原代人角膜表面细胞。经405 nm蓝光照射3min后，继续孵育24小时后，用四甲基偶氮唑蓝比色法检测存活细胞数。结果在亚汇流条件下，蓝光照射后细胞活力下降约50%，与未照射的对照细胞相比，细胞活力下降约50%。蓝光对细胞的光毒性无明显影响，但紫外光阻挡和蓝光阻挡对细胞的光毒性有保护作用，使存活细胞减少30%~40%(紫外线)和15%~30%(蓝光)。结论蓝光对眼表细胞有损伤作用，遮荫对细胞有保护作用。我们建议使用蓝光保护来保持眼睛健康，特别是在高危人群中，如干眼症患者、隐形眼镜使用者、营养不良者和老年人。
Objective Blue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin.Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours' incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades.Results Under subconfluent conditions, viable cells decreased by around 50% after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30%-40% reduction (ultraviolet) and 15%-30% reduction (blue light) in viable cells.Conclusion These results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.