Cardioprotective effect of hydrogen sulfide in ischemic reperfusion experimental rats and its influence on expression of survivin gene.
Cardioprotective effect of hydrogen sulfide in ischemic reperfusion experimental rats and its influence on expression of survivin gene.
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DOI：
10.1248/bpb.32.1406
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发表时间：
2009-08
影响因子：
2
通讯作者：
Y. Zhuo;Pei-fang Chen;A. Zhang;Han Zhong;Chang-qing Chen;Yi-Zhun Zhu
中科院分区：
文献类型：
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作者：
Y. Zhuo;Pei-fang Chen;A. Zhang;Han Zhong;Chang-qing Chen;Yi-Zhun Zhu
硫化氢（H（2）S）作为一种内源性气体信号分子，具有重要的生物学功能，可能在缺血再灌注（I/R）心肌中发挥保护作用。本实验观察了H（2）S对大鼠心肌缺血再灌注损伤的保护作用及其对凋亡抑制基因Survivin表达的影响。将动物随机分为3组，分别在术前1周每天给予溶剂、硫氢化钠（NaHS）或DL-炔丙基甘氨酸（PAG），并在I/R后继续治疗2 d直至处死动物。通过测定血浆硫化氢浓度、血压，结合心电图，探讨硫化氢对心功能的影响。我们还比较了各组的心肌梗死面积和抗凋亡基因生存素的表达。结果显示，NaHS组在血压和心电图方面有明显改善。NaHS组心肌梗死面积明显缩小，Survivin表达上调，与其他两组比较，也显示了H（2）S的心肌保护作用。
Hydrogen sulfide (H(2)S), as an endogenous gas signaling molecule with important biological function that has been found recently, may play a protection in ischemic reperfusion (I/R) myocardium. We investigated the cardioprotective effect of H(2)S in rats model of ischemic reperfusion in vivo and a probably influence on the expression of survivin, an anti-apoptosis gene. Animals were randomly divided into 3 groups and received either vehicle, sodium hydrosulfide (NaHS) or DL-propargylglycine (PAG) respectively everyday for 1 week before surgery and the treatment continued for a further 2 d after I/R till the animals were sacrificed. We investigated the plasma H(2)S concentration and blood pressure, with the electrocardiogram (ECG) together, to prove the effect of H(2)S to the heart function. We also compared the heart infarct size and the expression of an anti-apoptosis gene, survivin, among groups. As the data shown, the NaHS group had great improvement in blood pressure and electrocardiogram situation. And the remarkable shrink of the infarct size and up-regulation of survivin in NaHS group comparing with the other two groups also showed the cardio protective effect of H(2)S in our study.