Three-dimensional tissue models of normal and diseased skin.
Three-dimensional tissue models of normal and diseased skin.
复制标题
DOI：
10.1002/0471143030.cb1909s41
复制
发表时间：
2008-12
影响因子：
--
通讯作者：
Garlick, Jonathan A
中科院分区：
文献类型：
--
作者：
Carlson, Mark W;Alt-Holland, Addy;Egles, Christophe;Garlick, Jonathan A
在过去的十年中，在体外，人类，三维（3D）组织模型，被称为人类皮肤等效物（HSE）的发展，进一步了解表皮细胞生物学，并提供了新的实验系统。介导生长和分化之间联系的信号通路在细胞空间组织以显示体内可见的结构特征时发挥最佳作用，但在二维培养系统中解偶联并丢失。HSE由生长在空气-液体界面上的复层鳞状上皮组成，胶原基质上填充有真皮成纤维细胞。这些3D组织在体内表现出类似上皮的分化和形态，以及细胞分裂的速率，与人类皮肤中发现的相似。该单元描述了HSEs的制造，允许模拟人类皮肤的形态，分化和生长的人体组织的生成，以及癌症和伤口再上皮化的疾病过程，为人类疾病的研究提供了强大的新工具。
Over the last decade, the development of in vitro, human, three-dimensional (3D) tissue models, known as human skin equivalents (HSEs), has furthered understanding of epidermal cell biology and provided novel experimental systems. Signaling pathways that mediate the linkage between growth and differentiation function optimally when cells are spatially organized to display the architectural features seen in vivo, but are uncoupled and lost in two-dimensional culture systems. HSEs consist of a stratified squamous epithelium grown at an air-liquid interface on a collagen matrix populated with dermal fibroblasts. These 3D tissues demonstrate in vivo–like epithelial differentiation and morphology, and rates of cell division, similar to those found in human skin. This unit describes fabrication of HSEs, allowing the generation of human tissues that mimic the morphology, differentiation, and growth of human skin, as well as disease processes of cancer and wound re-epithelialization, providing powerful new tools for the study of diseases in humans.