Shock Wave Therapy Promotes Cardiomyocyte Autophagy and Survival during Hypoxia
Shock Wave Therapy Promotes Cardiomyocyte Autophagy and Survival during Hypoxia
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冲击波疗法促进缺氧期间心肌细胞自噬和存活。
DOI：
10.1159/000477885
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发表时间：
2017-01-01
影响因子：
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通讯作者：
He, Qing
中科院分区：
文献类型：
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作者：
Du, Ling;Shen, Tao;He, Qing
背景：自噬在心血管疾病中起着重要作用。关于自噬对缺血/缺氧心肌的影响仍存在争议。心脏冲击波疗法(CSWT)是治疗难治性缺血性心脏病的有效替代疗法。CSWT是否能调节低氧条件下心肌细胞的自噬尚不清楚。我们利用H9c2细胞建立了心肌缺氧模型，并进行了冲击波处理，以评价冲击波对自噬的影响。方法：H9c2细胞在低氧条件下孵育，然后以0.02、0.05、0.10mJ/mm(2)能量进行短波处理。检测细胞活力和细胞内三磷酸腺苷水平。免疫印迹法检测LC3B、AMPK、mTOR、Beclin-1、Sirt1和HIF-1α的表达。单丹西林染色可见自噬空泡。结果：缺氧24小时后，H9c2细胞的存活率和ATP水平显著降低，自噬功能明显增强。能量为0.05MJ/mm(2)的短波处理显著提高了细胞存活率、ATP水平、Lc3B-II/I和自噬空泡数量。此外，SW治疗后，磷酸化AMPK和Sirt1升高，磷酸化mTOR和HIF-1a降低。结论：SW治疗可能通过调节AMPK/mTOR通路，促进缺氧心肌细胞自噬，保护心肌细胞功能。(C)2017年作者(S)由S.Karger AG，巴塞尔出版
Background: Autophagy plays an important role in cardiovascular disease. Controversy still exists regarding the effect of autophagy on ischemic/hypoxic myocardium. Cardiac shock wave therapy (CSWT) is an effective alternative treatment for refractory ischemic heart disease. Whether CSWT can regulate cardiomyocyte autophagy under hypoxic conditions is not clear. We established a myocardial hypoxia model using the H9c2 cell line and performed shock waves (SWs) treatment to evaluate the effect of SW on autophagy. Methods: The H9c2 cells were incubated under hypoxic conditions, and SW treatment was then performed at energies of 0.02, 0.05, or 0.10 mJ/mm(2). The cell viability and intracellular ATP level were examined. Western blot analysis was used to assess the expression of LC3B, AMPK, mTOR, Beclin-1, Sirt1, and HIF-1 alpha. Autophagic vacuoles were visualized by monodansylcadaverine staining. Results: After the 24-hour hypoxic period, cardiomyocyte viability and ATP levels were decreased and autophagy was significantly increased in H9c2 cells. SW treatment with an energy of 0.05 mJ/mm(2) significantly increased the cellular viability, ATP level, LC3B-II/I, and number of autophagic vacuoles. In addition, phosphorylated AMPK and Sirt1 were increased and phosphorylated mTOR and HIF-1a were decreased after SW treatment. Conclusion: SW treatment can potentially promote cardiomyocyte autophagy during hypoxia and protect cardiomyocyte function by regulating the AMPK/mTOR pathway. (C) 2017 The Author(s) Published by S. Karger AG, Basel