Simultaneous determination of rifabutin and human serum albumin in pharmaceutical formulations by capillary electrophoresis
Simultaneous determination of rifabutin and human serum albumin in pharmaceutical formulations by capillary electrophoresis
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DOI：
10.1016/j.vascn.2017.01.003
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发表时间：
2017-05-01
影响因子：
1.9
通讯作者：
Gelperina, S.
中科院分区：
文献类型：
--
作者：
Ermolenko, Yu.;Anshakova, A.;Gelperina, S.
采用毛细管区带电泳（CZE）法测定了抗结核抗生素利法布里（RFB）在不同制剂中的含量。此外，同时测定RFB和人血清白蛋白（HSA）。在不同pH水平下检查RFB的电泳行为。CE条件：石英毛细管（内径75 mm，有效长度50 cm，总长度60 cm），毛细管温度25 ℃，施加于毛细管的电压为+20kV，紫外检测波长214 nm，样品在30 mbar下流动注射5s，四硼酸盐缓冲溶液（0.01M，pH9.2）。所得结果的特征在于高效（理论塔板数高达260，000）和足够的灵敏度（RFB的LOQ从0.02 μ g/ml开始）。所得数据与HPLC（RFB）和分光光度法（HSA）结果均雅阁。(C)2017爱思唯尔公司All rights reserved.
Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75mm, effective length 50 cm, total length 60 cm), the capillary temperaturewas 25 degrees C, the voltage applied to the capillary tube was+20 kV, the UV detection wavelength was 214 nm, hydrodynamic injection of the sample was performed at 30 mbar for 5 s, tetraborate buffer solution (0.01 M, pH 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02 mu g/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA). (C) 2017 Elsevier Inc. All rights reserved.