Identification and characterization of biocontrol agent Lysinibacillus boronitolerans P42 against Cerrena unicolor that causes root rot of arecanut palm
Identification and characterization of biocontrol agent Lysinibacillus boronitolerans P42 against Cerrena unicolor that causes root rot of arecanut palm
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DOI：
10.1007/s00203-023-03433-y
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发表时间：
2023-04
影响因子：
2.8
通讯作者：
Xiang Ma;Yuxiao Gao;Hong Li;Dan Wang;Juanjuan Li;Xin-wen Hu;Xi Huang;Min Lin;Yanqiong Tang;Zhu Liu
中科院分区：
文献类型：
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作者：
Xiang Ma;Yuxiao Gao;Hong Li;Dan Wang;Juanjuan Li;Xin-wen Hu;Xi Huang;Min Lin;Yanqiong Tang;Zhu Liu
槟榔是世界热带地区最重要的经济作物之一。槟榔根腐病是槟榔生产中的一个重要病害，严重影响了槟榔产业的发展，尤其是海南省的槟榔生产。化学药剂的普遍使用加剧了抗性病原菌的产生和农业环境的污染问题。本研究旨在筛选和鉴定一种更有效、更环保的防治槟榔根腐病的生物防治方法。通过菌丝生长速率的测定，筛选出对土传病原菌具有较强抗性的拮抗细菌，其中拮抗效果最强的菌株P42（82.18%）。经16 s rDNA序列分析，鉴定菌株P42为耐硼赖氨酸芽孢杆菌（Lysinibacillus boronitolerans）。P42菌株对多种热带农业病原真菌具有广谱的拮抗活性，包括Cerrena unicolor、Magnaporthe oryzea、Botryodiplodia theobromae、Neoscytalidium dimidiatum、Thanatephorus cucumeris、Fusarium oxysporum和Botrytis cinereaPer. P42的培养物的拮抗活性耐受常见的蛋白酶，较长的储存时间，温度范围为40-121 °C;并且受碱性（7-9）和酸性（1-2）pH以及紫外线处理30分钟以上的显著影响。发酵滤液粗提物的抑菌活性研究表明，其活性成分可能是脂肽、聚酮或蛋白质。据我们所知，这是L.硝酸硼是防治槟榔根腐病的潜在生物制剂。
The arecanut palm is one of the most important industrial crops in tropical area around the world. The root rot of arecanut palm, which is caused byCerrena unicolor, has led to heavy economic losses and restricted greatly the development of arecanut industry, especially in Hainan province of China. The common use of chemical agents has worsened the problems of the emergence of resistant pathogens and the pollution of agricultural environment. This study aims to screen and identify a more effective and environment friendly biocontrol method for the prevention and treatment of root rot of arecanut palm. The mycelium growth rate is investigated to select antagonistic bacteria from tropical crop rotation fields which show improved resistance against soil-borne pathogens, and the strain P42 is revealed with the strongest antagonistic effects (82.18%). Based on 16 s rDNA sequence analysis, the strain P42 is identified asLysinibacillus boronitolerans. In vitro antimicrobial activity shows that the strain P42 exhibits broad-spectrum antagonistic activity against a wide variety of tropical agricultural fungal pathogens, includingCerrena unicolor,Magnaporthe oryzea,Botryodiplodia theobromae,Neoscytalidium dimidiatum,Thanatephorus cucumeris,Fusarium oxysporum, andBotrytis cinereaPer.. The antagonistic activity of the culture of P42 is tolerant to common proteases, longer storage time, and temperature range of 40–121 °C; and is significantly influenced by alkaline (7–9) and acidic (1–2) pH, as well as by ultraviolet ray treatment for more than 30 min. The investigation on the antagonistic activity of the crude extract of fermentation filtrate indicates that the active compounds might be lipopeptides, polyketones, or proteins. To our knowledge, this is the first report ofL. boronitoleransas potential bio-reagents for controlling root rot of arecanut palm caused byCerrena unicolor.