THE RCC1 PROTEIN INTERACTS WITH RAN, RANBP1, HSC70, AND A 340-KDA PROTEIN IN XENOPUS EXTRACTS
THE RCC1 PROTEIN INTERACTS WITH RAN, RANBP1, HSC70, AND A 340-KDA PROTEIN IN XENOPUS EXTRACTS
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DOI：
10.1074/jbc.270.18.10658
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发表时间：
1995-05-05
影响因子：
4.8
通讯作者：
DASSO, M
中科院分区：
文献类型：
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作者：
SAITOH, H;DASSO, M
RCC 1是一种丰富的、高度保守的染色质相关蛋白，其功能是维持正常有序的细胞周期所必需的，RCC 1也是许多核过程所必需的，包括核转运和RNA代谢;并且它作为一种称为Ran的小的、ras相关的GTdR的鸟嘌呤核苷酸交换因子酶促发挥作用。对几种生物的研究表明，RCC 1可能是含有多种蛋白质的大型复合物的一部分。还有证据表明，RCC 1通过其他蛋白质与染色质结合，并且复合物与染色质的结合在细胞周期内变化。为了表征这种假定的复合物，我们已经通过它们与GST-RCC 1融合蛋白的结合鉴定了许多其他蛋白作为复合物的候选组分。这些蛋白质中的三种以前已经被鉴定（Ran、RanBP 1和hsc 70）。第四种蛋白质是新的，分子量为340 kDa。在这份报告中，我们讨论了这些蛋白质之间的相互作用的初步表征。
RCC1 is an abundant, highly conserved, chromatin-associated protein whose function is necessary for the preservation of a properly ordered cell cycle, RCC1 is also necessary for numerous nuclear processes, including nuclear transport and RNA metabolism; and it functions enzymatically as a guanine nucleotide exchange factor for a small, ras related GTPase called Ran. Studies in several organisms suggest that RCC1 may be part of a large complex containing multiple proteins. There is also evidence that RCC1 associates with chromatin through other proteins and that the binding of the complex to chromatin varies within the cell cycle. In order to characterize this putative complex, we have identified a number of other proteins as candidate compo nents of the complex by their association with a GST-RCC1 fusion protein. Three of these proteins have previously been identified (Ran, RanBP1, and hsc70). The fourth protein is novel and has a molecular mass of 340 kDa. In this report, we discuss a preliminary characterization of the interactions between these proteins.