Sensitive detection of whole-genome differentiation among closely-related species of the genus Fusarium using DNA-DNA hybridization and a microplate technique.
Sensitive detection of whole-genome differentiation among closely-related species of the genus Fusarium using DNA-DNA hybridization and a microplate technique.
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DOI：
10.1292/jvms.12-0034
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发表时间：
2012-05
期刊：
影响因子：
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通讯作者：
M. Watanabe;K. Goto;Y. Sugita‐Konishi;Y. Kamata;Y. Hara-Kudo
中科院分区：
文献类型：
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作者：
M. Watanabe;K. Goto;Y. Sugita‐Konishi;Y. Kamata;Y. Hara-Kudo
我们开发了一个新的系统，用于检测全基因组分化DNA-DNA杂交，并测试了其敏感性与三个密切相关的镰刀菌物种。我们比较了DNA-DNA相关的核苷酸序列同源性的5个基因区域之间的每一个菌株的3个镰刀菌。DNA-DNA的相关性为16.2- 86.6%。18 S rDNA、ITS 1到28 S rDNA的rDNA簇区、β-tub、EF-1α和lys 2的同源性分别为100.0、99.0-100.0、96.7-100.0、95.1-99.4和94.7- 100.0%。我们的系统可以清楚地检测具有非常相似的形态特征的密切相关的真菌物种之间的差异，并且在核苷酸序列中表现出很少的诊断。我们的研究结果表明，该系统是一个很好的工具，近缘真菌物种的鉴定和系统发育分析。
We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2-86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0-100.0, 96.7-100.0, 95.1-99.4, and 94.7-100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.