INVIVO PROTOCOL FOR TESTING EFFICACY OF HAND-WASHING AGENTS AGAINST VIRUSES AND BACTERIA - EXPERIMENTS WITH ROTAVIRUS AND ESCHERICHIA-COLI
INVIVO PROTOCOL FOR TESTING EFFICACY OF HAND-WASHING AGENTS AGAINST VIRUSES AND BACTERIA - EXPERIMENTS WITH ROTAVIRUS AND ESCHERICHIA-COLI
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DOI：
10.1128/aem.55.12.3113-3118.1989
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发表时间：
1989-12-01
影响因子：
4.4
通讯作者：
TOSTOWARYK, W
中科院分区：
文献类型：
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作者：
ANSARI, SA;SATTAR, SA;TOSTOWARYK, W
比较了十种防腐剂配方、一种不含药物的液体肥皂和自来水对成年志愿者指垫上的轮状病毒的清除能力；其中三种防腐剂、肥皂和自来水也分别被检测是否有大肠杆菌。将病毒或细菌的粪便悬浮液放在每个指垫上并风干。受污染的地点暴露在测试产品中10秒，用自来水冲洗，并用纸巾擦干。然后将残留的病毒或细菌洗脱。选定的药剂也通过一种类似的全手方法进行测试，通过这种方法，双手的整个手掌表面都被污染了。单独使用酒精（70%）或与沙夫隆（Savlon）一起使用，可使病毒滴度降低50%至99%，而普罗维定、滴露和Hibisol的降低幅度为95%至97%。葡萄糖酸氯己定水溶液对病毒的去除或灭活效果明显低于70%酒精溶液。此外，在水中（1:200）的沙夫隆被发现对病毒（80.6%）的去除效果远低于细菌（98.9%）。自来水和肥皂分别使病毒滴度降低83.6%和72.5%，细菌滴度降低90%和68.7%。全手法与指垫法的结果吻合较好。我们认为手指垫法是一种适合测试洗手剂体内功效的模型，并强调使用适当的测试病毒和细菌的必要性。
Ten antiseptic formulation, an unmedicated liquid soap, and tap water alone were compared for their capacities to eliminate human rotavirus from the finger pads of adult volunteers; three of the antiseptics, the soap, and the tap water alone were also tested against Escherichia coli. A fecal suspension of virus or bacterium was placed on each finger pad and air dried. The contaminated site was exposed to the test product for 10 s, rinsed in tap water, and dried on a paper towel. The residual virus or bacterium was then eluted. Selected agents were also tested by a analogous whole-hand method by which the entire palm surfaces of both hands were contaminated. Alcohols (70%) alone or with Savlon reduced the virus titer by > 99%, whereas the reductions by Proviodine, Dettol, and Hibisol ranged from 95 to 97%. Aqueous solutions of chlorhexidine gluconate were significantly less effective for virus removal or inactivation than 70% alcohol solutions. Furthermore, Savlon in water (1:200) was found to be much less effective in eliminating the virus (80.6%) than the bacterium (98.9%). The tap water alone and the soap reduced the virus titers by 83.6 and 72.5% and the bacterial titers by 90 and 68.7%, respectively. The results of the whole-hand method agreed well with those of the finger pad protocol. We conclude that the finger pad method is a suitable model for testing the in vivo efficacy of hand-washing agents and emphasize the need for using appropriate test viruses and bacteria.