Forskolin and phorbol esters reduce the same potassium conductance of mouse neurons in culture.
Forskolin and phorbol esters reduce the same potassium conductance of mouse neurons in culture.
复制标题
毛喉素和佛波酯降低培养物中小鼠神经元的相同钾电导。
DOI：
10.1126/science.2432663
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Macdonald,RL
中科院分区：
文献类型：
--
作者：
Grega,DS;Werz,MA;Macdonald,RL
第二信使系统可以通过蛋白质磷酸化调节神经元活动。然而，两个主要的第二信使途径（环磷酸腺苷和磷脂酰肌醇系统）之间的相互作用尚不清楚。研究了环AMP依赖性蛋白激酶和蛋白激酶C激活剂对原代分离细胞培养物中生长的小鼠背根神经节神经元和大脑半球神经元的静息膜特性、动作电位和电流的影响。毛喉素 (FOR) 和佛波醇 12,13-二丁酸酯 (PDBu) 均不会改变静息膜特性，但都会增加中枢和外周神经元钙依赖性动作电位的持续时间。通过单电极电压钳技术，FOR 和 PDBu 可降低相同的电压依赖性钾电导。这表明两个独立的第二信使系统可能影响相同的钾电导。
Second messenger systems may modulate neuronal activity through protein phosphorylation. However, interactions between two major second messenger pathways, the cyclic AMP and phosphatidylinositol systems, are not well understood. The effects of activators of cyclic AMP-dependent protein kinase and protein kinase C on resting membrane properties, action potentials, and currents recorded from mouse dorsal root ganglion neurons and cerebral hemisphere neurons grown in primary dissociated cell culture were investigated. Neither forskolin (FOR) nor phorbol 12,13-dibutyrate (PDBu) altered resting membrane properties but both increased the duration of calcium-dependent action potentials in both central and peripheral neurons. By means of the single-electrode voltage clamp technique, FOR and PDBu were shown to decrease the same voltage-dependent potassium conductance. This suggests that two independent second messenger systems may affect the same potassium conductance.