Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone.
Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone.
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DOI：
10.1210/endo.134.1.8275945
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发表时间：
1994
期刊：
影响因子：
4.8
通讯作者：
Su‐Li Cheng;J. Yang;L. Rifas;Shu-Fang Zhang;L. Avioli
中科院分区：
文献类型：
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作者：
Su‐Li Cheng;J. Yang;L. Rifas;Shu-Fang Zhang;L. Avioli
在体外细胞培养系统中检测人骨髓基质细胞的成骨潜能。地塞米松（Dex）治疗诱导这些细胞从细长的形态转化为一个更立方形的形状，增加他们的碱性磷酸酶活性和cAMP的PTH和前列腺素E2的反应，是必不可少的矿化的细胞外基质。Dex诱导的人骨髓基质细胞分化在治疗2-3天后明显，并在7-14天达到最大值，根据碱性磷酸酶活性判断，尽管Dex减弱了1，25-二羟维生素D3对骨钙素的诱导。Dex的撤回导致1，25-二羟维生素D3诱导的骨钙素分泌的增强，而碱性磷酸酶活性和cAMP对PTH的反应保持在撤回前的水平。Dex对骨连接素的稳态mRNA水平无影响。我们的研究结果表明，地塞米松的条件下，人骨髓成骨基质细胞分化成成骨细胞样细胞，支持糖皮质激素在确保成熟的成骨细胞群体的充足供应的许可效应的假设。此外，建立的人骨髓基质细胞培养体系为研究人骨成骨祖细胞的分化调控提供了良好的体外模型。
Human bone marrow stromal cells were examined for their osteogenic potential in an in vitro cell culture system. Dexamethasone (Dex) treatment induced morphological transformation of these cells from an elongated to a more cuboidal shape, increased their alkaline phosphatase activity and cAMP responses to PTH and prostaglandin E2, and was essential for mineralization of the extracellular matrix. Dex-induced differentiation of human bone marrow stromal cells was apparent after 2-3 days of treatment and reached a maximum at 7-14 days, as judged by alkaline phosphatase activity, although induction of osteocalcin by 1,25-dihydroxyvitamin D3 was attenuated by Dex. Withdrawal of Dex resulted in an enhancement of the 1,25-dihydroxyvitamin D3-induced secretion of osteocalcin, whereas alkaline phosphatase activity and the cAMP response to PTH remained at prewithdrawal levels. The steady state mRNA level of osteonectin was not affected by Dex. Our results, which demonstrate that Dex conditions the differentiation of human bone marrow osteogenic stromal cells into osteoblast-like cells, support the hypothesis of a permissive effect of glucocorticoids in ensuring an adequate supply of mature osteoblast populations. Furthermore, the established human bone marrow stromal cell culture provides a good model of an in vitro system to study the regulation of differentiation of human bone osteoprogenitor cells.