Cortistatin promotes and negatively correlates with slow-wave sleep
Cortistatin promotes and negatively correlates with slow-wave sleep
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DOI：
10.1111/j.1460-9568.2007.05696.x
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发表时间：
2007-08-01
影响因子：
3.4
通讯作者：
de Lecea, Luis
中科院分区：
文献类型：
--
作者：
Bourgin, Patrice;Fabre, Veronique;de Lecea, Luis
睡眠需求的特征在于慢波活动（SWA）的水平，并随着清醒时间的增加而增加。这种睡眠自我平衡过程的分子本质实际上是未知的。在这里，我们表明，脑室内给药的神经肽，皮质抑素（CST-14），增强脑电同步，选择性地促进深慢波睡眠（SWS）在光明和黑暗的时期在大鼠。CST-14还在CST-14给药后的前两个小时内增加深SWS内的慢波活动（SWA）水平。前皮质抑素原mRNA的稳态水平在光暗周期中振荡，在24小时睡眠剥夺后高出4倍，在8小时睡眠恢复后逐渐恢复到正常水平。前皮质抑素原mRNA在睡眠剥夺后在与c-fos共定位的皮质中间神经元的特定子集中表达。与此相反，CST阳性细胞的数量减少，共表达pERK 1/2睡眠剥夺。CST-14增加SWA的能力，以及前皮质抑素原与SWS中花费的时间的负相关性，表明在睡眠稳态过程中的潜在作用。
Sleep need is characterized by the level of slow-wave activity (SWA) and increases with time spent awake. The molecular nature of this sleep homeostatic process is practically unknown. Here, we show that intracerebroventricular administration of the neuropeptide, cortistatin (CST-14), enhances EEG synchronization by selectively promoting deep slow-wave sleep (SWS) during both the light and dark period in rats. CST-14 also increases the level of slow-wave activity (SWA) within deep SWS during the first two hours following CST-14 administration. Steady-state levels of preprocortistatin mRNA oscillate during the light : dark cycle and are four-fold higher upon total 24-h sleep deprivation, returning progressively to normal levels after eight hours of sleep recovery. Preprocortistatin mRNA is expressed upon sleep deprivation in a particular subset of cortical interneurons that colocalize with c-fos. In contrast, the number of CST-positive cells coexpressing pERK1/2 decreases under sleep deprivation. The capacity of CST-14 to increase SWA, together with preprocortistatin's inverse correlation with time spent in SWS, suggests a potential role in sleep homeostatic processes.