TRANSCRIPTION OF A REGION DOWNSTREAM FROM LAMBDA-ORI IS REQUIRED FOR REPLICATION OF PLASMIDS DERIVED FROM COLIPHAGE LAMBDA
TRANSCRIPTION OF A REGION DOWNSTREAM FROM LAMBDA-ORI IS REQUIRED FOR REPLICATION OF PLASMIDS DERIVED FROM COLIPHAGE LAMBDA
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DOI：
10.1007/bf00332239
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发表时间：
1989-03-01
期刊：
影响因子：
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通讯作者：
MASAMUNE, Y
中科院分区：
文献类型：
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作者：
HASE, T;NAKAI, M;MASAMUNE, Y
λ噬菌体的DNA复制依赖于RNA聚合酶在λ ori区或其周围的转录激活。为了阐明转录激活的功能，我们构建了几个携带λ ori和lacP的质粒，它们的相对位置和方向彼此不同，并研究了这些重组质粒的复制活性。发现在紧邻λ ori下游的区域中的转录，而不是在λ pri区域中的转录，对于质粒复制是必需的。转录过程中需要一定的最小长度，只有向右定向转录是有效的激活。
DNA replication of lambda phage depends on transcriptional activation at or around the .lambda.ori region by RNA polymerase. To elucidate the function of the transcriptional activation, we constructed several plasmids carrying .lambda.ori and lacP, whose relative locations and directions were different from each other, and studied replication activity of these recombinant plasmids. Transcription in a region immediately downstream from .lambda.ori, but not in the .lambda.pri region, was found to be essential for plasmid replication. Transcription proceeding over a certain minimal length was required and only rightward-directed transcription was effective for the activation.