DOPA DECARBOXYLASE ACTIVITY OF THE LIVING HUMAN BRAIN
DOPA DECARBOXYLASE ACTIVITY OF THE LIVING HUMAN BRAIN
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DOI：
10.1073/pnas.88.7.2721
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发表时间：
1991-04-01
影响因子：
11.1
通讯作者：
KUWABARA, H
中科院分区：
文献类型：
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作者：
GJEDDE, A;REITH, J;KUWABARA, H
单胺能神经元使用多巴脱羧酶（DDC;芳香族-L-氨基酸羧基裂解酶，EC 4.1.1.28）从L-3，4-二羟基苯丙氨酸（L-多巴）形成多巴胺。我们用6-[F-18]fluoro-L-dopa和正电子发射断层扫描仪测量了6名健康志愿者脑中的多巴脱羧酶活性。我们使用动力学模型计算了相对于其K（m）的酶活性，该模型得出了6-[F-18]氟-L-多巴转化为[F-18]氟多巴胺的相对转化率。相对多巴脱羧酶活性的区域值范围从零在枕叶皮质到1.9 h-1在尾状核和壳核，在协议中获得的值在体外。
Monoaminergic neurons use dopa decarboxylase (DDC; aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) to form dopamine from L-3,4-dihydroxyphenylalanine (L-dopa). We measured regional dopa decarboxylase activity in brains of six healthy volunteers with 6-[F-18]fluoro-L-dopa and positron emission tomography. We calculated the enzyme activity, relative to its K(m), with a kinetic model that yielded the relative rate of conversion of 6-[F-18]fluoro-L-dopa to [F-18]fluorodopamine. Regional values of relative dopa decarboxylase activity ranged from nil in occipital cortex to 1.9 h-1 in caudate nucleus and putamen, in agreement with values obtained in vitro.