Direct evidence for the intact hepatocyte theory in patients with liver cirrhosis.
Direct evidence for the intact hepatocyte theory in patients with liver cirrhosis.
复制标题
肝硬化患者完整肝细胞理论的直接证据。
DOI：
10.1016/0016-5085(92)90775-t
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发表时间：
1992
期刊：
影响因子：
29.4
通讯作者：
Y. Idezuki
中科院分区：
文献类型：
--
作者：
S. Kawasaki;H. Imamura;Y. Bandai;K. Sanjo;Y. Idezuki
试图比较各种形态参数，包括总肝细胞数，在体内清除氨基比林和安替比林在26例肝硬化和14例nonbacterial患者，以评估完整的肝细胞理论。采用计算机辅助彩色图像分析仪，采用新开发的方法进行形态学分析。在所有患者中，氨基比林清除率与肝体积（r= 0.434;P< 0.05）、实质细胞体积（r= 0.574;P< 0.001）显著相关，与肝细胞总数（r= 0.614;P< 0.001）最强。三个参数与安替比林清除率呈显著正相关（r= 0.367，P < 0.05;r= 0.663，P < 0.001; r = 0.807，P < 0.001）。肝硬化患者和非肝硬化患者的单个肝细胞对氨基比林的平均清除率无显著性差异（分别为3.52 ± 1.60 × 10- 10 mL/min和3.65 ± 1.50 × 10- 10 mL/min;P> 0.10）。安替比林/肝细胞的清除率也相似（抗肿瘤药为7.35 ± 2.27 × 10- 10 mL/min，非抗肿瘤药为6.16 ± 1.07 × 10- 10 mL/min;P> 0.10）。因此，第一次直接评价了最初在完整细胞假设中提出的每个肝细胞的药物固有清除率，为完整肝细胞理论提供了强有力的支持。
An attempt was made to compare various morphometric parameters, including total hepatocyte number, with the in vivo clearances of aminopyrine and antipyrine in 26 cirrhotic and 14 noncirrhotic patients to evaluate the intact hepatocyte theory. Morphometric analysis was performed with a newly developed method using a computer-aided color image analyzer. Aminopyrine clearance was significantly correlated with liver volume (r= 0.434;P< 0.05), parenchymal cell volume (r= 0.574;P< 0.001), and most strongly with total hepatocyte number (r= 0.614;P< 0.001) in all patients. Significant correlations were also observed between these three parameters and antipyrine clearance (r= 0.367,P< 0.05;r= 0.663,P< 0.001; andr= 0.807,P< 0.001, respectively). The mean aminopyrine clearance per individual hepatocyte showed no significant difference between cirrhotic and noncirrhotic patients (3.52 ± 1.60 × 10-10mL/min vs. 3.65 ± 1.50 × 10-10mL/min, respectively;P> 0.10). Similar results were obtained for antipyrine clearance per hepatocyte (7.35 ± 2.27 × 10-10mL/min for cirrhotics vs. 6.16 ± 1.07 × 10-10mL/min for noncirrhotics;P> 0.10). Thus, the intrinsic clearances of drugs per individual hepatocyte, as originally proposed in the intact cell hypothesis, were directly evaluated for the first time, lending strong support to the intact hepatocyte theory.